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Oligo Synthesis

Oligo Synthesis : CEPs

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Br-U-CE Phosphoramidite

Br-U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3090-02Br-U-CE Phosphoramidite 0.25g £380.00£361.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-3090-90Br-U-CE Phosphoramidite 100µmoles £156.00£148.20Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-3090-95Br-U-CE Phosphoramidite 50µmoles £78.40£74.48Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Br-U-CE Phosphoramidite

Br-U-CE Phosphoramidite

Glen Research

Br-U-CE Phosphoramidite

Structure

Catalog Number: 10-3090-xx

Description: Br-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-bromo-Uridine,2'-O-TBDMS,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C45H60N4O9BrPSi M.W.: 939.96 F.W.: 385.06

Diluent: Anhydrous Acetonitrile
Coupling: 12 minutes
Deprotection: Ammonium hydroxide/Ethanol 3:1 (v/v) at room temperature as required by the nucleobases. Complete the 2' deprotection using standard RNA protocols.  Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

minor rna phosphoramidites (TBDMS PROTECTED)

Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments. 6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used. 2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

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Br-U-CE Phosphoramidite

Br-U-CE Phosphoramidite

Glen Research

Br-U-CE Phosphoramidite

Br-U-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3090-02 0.25grams .25grams 2.66 75.33 45.2 28.25 20.55 15.07 3.77
10-3090-90 100µmoles .094grams 1 20 12 7.5 5.45 4 1
10-3090-95 50µmoles .047grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3090-02 0.25grams .25grams 3.97 .07 N/A N/A 15.21 N/A
10-3090-90 100µmoles .094grams 1.5 .07 N/A N/A 4.92 N/A
10-3090-95 50µmoles .047grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3090-02 0.25grams .25grams 3.97 .07 N/A 12.03 11.3

10-3090-90 100µmoles .094grams 1.5 .07 N/A 4.06 3.82

10-3090-95 50µmoles .047grams .75 .07 N/A 1.65 1.55

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200