Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Reagents

Prices quoted are for single packs only.

For multiples of the same product please request a quote.

Some of Glen's products are hazardous and may be subject to additional shipping charges.

Full product information is available on Glen Research's web site.

Azidobutyrate NHS Ester

Azidobutyrate NHS Ester

Glen Research

Catalogue No.DescriptionPack SizePriceQty
50-1904-23Azidobutyrate NHS Ester 2.3mg £48.00£44.16Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
50-1904-24Azidobutyrate NHS Ester 23mg £240.00£220.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Azidobutyrate NHS Ester

Azidobutyrate NHS Ester

Glen Research

Azidobutyrate NHS Ester

Structure

Catalog Number: 50-1904-xx

Description: Azidobutyrate NHS Ester

4-Azido-butan-1-oic acid N-Hydroxysuccinimide Ester
Formula: C8H10N4O4 M.W.: 226.19 F.W.: 113.12

Diluent: Anhydrous ACN, DMSO or DMF
Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt.
Deprotection: Not Applicable
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Azidobutyrate NHS Ester

Azidobutyrate NHS Ester

Glen Research

MSDS

Glen Report 20.1: MORE CLICK CHEMISTRY
Glen Report 22.2: Technical Brief - Crosslinking with Click Chemistry

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Azidobutyrate NHS Ester

Azidobutyrate NHS Ester

Glen Research

All products of baseclick are patent protected and available in collaboration with baseclick.
Baseclick GmbH has filed the following patent applications:
1. WO2006/117161, New labelling strategies for the sensitive detection of analytes
2. WO2008/952775, Click Chemistry for the production of reporter molecules

Baseclick GmbH holds a worldwide license for the research market of the “Click Chemistry” patent from “The Scripps Research Institute”:
3. WO03/101972, Copper-catalysed ligation of azides and acetylenes

(1) R. Kumar, et al., Journal of the American Chemical Society, 2007, 129, 6859-6864.
(2) J. Lietard, A. Meyer, J.J. Vasseur, and F. Morvan, Tetrahedron Letters, 2007, 48, 8795-8798.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Azidobutyrate NHS Ester

Azidobutyrate NHS Ester

Glen Research

Azidobutyrate NHS Ester

4-Azido-butan-1-oic acid N-Hydroxysuccinimide Ester
Formula: C8H10N4O4 M.W.: 226.19 F.W.: 113.12

Diluent: Anhydrous ACN, DMSO or DMF
Coupling: For labeling 0.2 umoles of an amino-modified oligonucleotide, dissolve 2.3 mg of Azidobutyrate NHS Ester in 60 uL of ACN (DMSO or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500 uL of 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12 uL of the Azidobutyrate NHS Ester solution to the dissolved oligonucleotide and let react for 2 hours at room temperature and desalt.
Deprotection: Not Applicable
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Material Safety Data Sheet

Literature Highlights

Glen Report 20.1: MORE CLICK CHEMISTRY

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200