Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

ATG Trimer Phosphoramidite

ATG Trimer Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
13-1032-90ATG Trimer Phosphoramidite 100µmoles £680.00£646.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
13-1032-95ATG Trimer Phosphoramidite 50µmoles £340.00£323.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

ATG Trimer Phosphoramidite

ATG Trimer Phosphoramidite

Glen Research

ATG Trimer Phosphoramidite

13-1032

Catalog Number: 10-1032-xx

Description: O4-Me-dT-CE Phosphoramidite

5'-Dimethoxytrityl-O4-methyl-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C41H51N4O8P M.W.: 758.85 F.W.: 318.22

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer. Monomers that allow for UltraMILD deprotection are recommended (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.) To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: If UltraMild Monomers were used: 0.05M Potassium Carbonate in Methanol, 4hrs at Room Temp.DMT-Off Synthesis: Add 6uL acetic acid per mL 0.05M potassium carbonate in methanol. Dry under vacuum. Desalt per standard procedures. DMT-On Synthesis: Use a Glen-Pak Purification Cartridge (60-5100-XX/60-5200-XX) to purify and desalt the oligonucleotide. Dilute the 0.05M potassium carbonate in methanol with 100mg/mL Sodium Chloride to a final concentration of 1:4(v/v). Load onto the prepped Glen-Pak Cartridge and purify as normal. If Standard Monomers were used: 1) Prepare a solution of 10% DBU in anhydrous methanol. DBU (1,8-Diazabicyclo[5.4.0]undec-7-ene) is available from Aldrich (139009-25G) as is anhydrous methanol (322415-100ML). 2) Transfer the CPG to a clean, dry glass vial and add 1mL of the 10% DBU solution. 3) Seal the vial and leave for 5 days in the dark at room temperature. 4) Transfer the contents to a micro centrifuge tube and reduce under vacuum to a small volume. 5) Add 1mL of 10mM aqueous sodium hydroxide solution and desalt or purify the oligonucleotide using standard procedures. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

MUTAGENESIS

Cellular polynucleotides are alkylated by endogenous components, such as S-adenosylmethionine, or after reacting with two general classes of environmental and laboratory chemicals. SN1 chemical agents include alkylnitrosourea and N-alkyl-N-nitro-N-nitrosoguanidine that react with the N7 position of guanine, N3 of adenine, O6 of guanine, O2 or O4 of pyrimidines, and the non-phosphodiester oxygen atoms of the phosphate backbone. In contrast, SN2 chemical agents such as methyl methanesulfonate and dimethyl sulfate react primarily with the N1 position of adenine (1-Methyl-2’-deoxyadenosine) and N3 of cytosine. To avoid chain branching during synthesis when using DCI as activator, N6-Me-dA is offered with acetyl protection.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ATG Trimer Phosphoramidite

ATG Trimer Phosphoramidite

Glen Research

MSDS

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ATG Trimer Phosphoramidite

ATG Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1032-02 0.25grams .25grams 3.29 96.33 57.8 36.13 26.27 19.27 4.82
10-1032-90 100µmoles .076grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1032-02 0.25grams .25grams 4.92 .07 92 57.5 41.82 5.75
10-1032-90 100µmoles .076grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1032-02 0.25grams .25grams 4.92 .07 93.6 58.5 42.55

10-1032-90 100µmoles .076grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200