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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

ATC Trimer Phosphoramidite

ATC Trimer Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
13-1031-90ATC Trimer Phosphoramidite 100µmoles £680.00£646.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
13-1031-95ATC Trimer Phosphoramidite 50µmoles £340.00£323.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

ATC Trimer Phosphoramidite

ATC Trimer Phosphoramidite

Glen Research

ATC Trimer Phosphoramidite

Structure

Catalog Number: 10-1031-xx

Description: 5'-OMe-dT-CE Phosphoramidite

5'-O-methyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-
phosphoramidite
Formula: C20H33N4O6P M.W.: 456.48 F.W.: 318.22

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.(Note: this product is a 5'-terminator.)
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

chain terminators

In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

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ATC Trimer Phosphoramidite

ATC Trimer Phosphoramidite

Glen Research

MSDS

Glen Report 20.1: Technical Brief - New application for 5Õ-OMe-dT phosphoramidite

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

ATC Trimer Phosphoramidite

ATC Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1031-02 0.25grams .25grams 5.48 169.33 101.6 63.5 46.18 33.87 8.47
10-1031-90 100µmoles .046grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1031-02 0.25grams .25grams 8.17 .07 157 98.13 71.36 9.81
10-1031-90 100µmoles .046grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1031-02 0.25grams .25grams 8.17 .07 158.6 99.13 72.09

10-1031-90 100µmoles .046grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200