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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-I-dT-CE Phosphoramidite

5'-I-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1931-025'-I-dT-CE Phosphoramidite 0.25g £236.00£224.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1931-905'-I-dT-CE Phosphoramidite 100µmoles £68.00£64.60Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

5'-I-dT-CE Phosphoramidite

5'-I-dT-CE Phosphoramidite

Glen Research

5'-I-dT-CE Phosphoramidite

Structure

Catalog Number: 10-1931-xx

Description: 5'-I-dT-CE Phosphoramidite

5'-Iodo-2'-deoxyThymidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C19H30N4O5PI M.W.: 552.35 F.W.: 414.09

Diluent: Anhydrous Acetonitrile
Coupling: Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature. (Deprotection may be carried out at room temperature in ammonium hydroxide for 24hr, however ~10% loss in iodine label will occur.)
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-I-dT-CE Phosphoramidite

5'-I-dT-CE Phosphoramidite

Glen Research

5'-I-dT-CE Phosphoramidite

5'-I-dT-CE Phosphoramidite

Glen Research

Conjugation with a Click

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1931-02 0.25grams .25grams 4.53 137.67 82.6 51.63 37.55 27.53 6.88
10-1931-90 100µmoles .055grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1931-02 0.25grams .25grams 6.76 .07 128.8 80.5 58.55 8.05
10-1931-90 100µmoles .055grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1931-02 0.25grams .25grams 6.76 .07 130.4 81.5 59.27

10-1931-90 100µmoles .055grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200