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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-Hexynyl Phosphoramidite

5'-Hexynyl Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1908-025'-Hexynyl Phosphoramidite 0.25g £160.00£152.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1908-905'-Hexynyl Phosphoramidite 100µmoles £48.00£45.60Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

5'-Hexynyl Phosphoramidite

5'-Hexynyl Phosphoramidite

Glen Research

5'-Hexynyl Phosphoramidite

Structure

Catalog Number: 10-1908-xx

Description: 5'-Hexynyl Phosphoramidite

6-Hexyn-1-yl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C15H27N2O2P M.W.: 298.36 F.W.: 160.11

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable to standard deprotection conditions and exhibit a slightly increased retention time on RP HPLC. Azides are not compatible with oligonucleotide synthesis using phosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHS Ester is used1 for azido-modification of amines at either the 3’-end or the 5’-end of an oligo and it can even be used for internal modification on an Amino-Modifier-C6 dX residue within the sequence. Specific to the 5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. This modifier can then be easily transformed into a 5’-azido group by displacement of bromide using sodium azide.2 Alkyne NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides as well as peptides or proteins. We also offer a synthesis support for labelling the 3’ terminus of oligonucleotides with an alkyne group for use in Click Chemistry. This builds upon our 1,3-diol product portfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide (prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding 5’-azide.

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5'-Hexynyl Phosphoramidite

5'-Hexynyl Phosphoramidite

Glen Research

MSDS

Glen Report 19.1

Glen Report 20.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Hexynyl Phosphoramidite

5'-Hexynyl Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1908-90 100µmoles .03grams 1 20 12 7.5 5.45 4 1
10-1908-02 0.25grams .25grams 8.38 266 159.6 99.75 72.55 53.2 13.3
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1908-90 100µmoles .03grams 1.5 .07 23.6 14.75 10.73 1.48
10-1908-02 0.25grams .25grams 12.51 .07 243.8 152.38 110.82 15.24
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1908-90 100µmoles .03grams 1.5 .07 25.2 15.75 11.45

10-1908-02 0.25grams .25grams 12.51 .07 245.4 153.38 111.55

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200