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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-Dabsyl-dT-CE Phosphoramidite

5'-Dabsyl-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1532-025'-Dabsyl-dT-CE Phosphoramidite 0.25g £620.00£570.40Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1532-905'-Dabsyl-dT-CE Phosphoramidite 100µmoles £200.00£184.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5'-Dabsyl-dT-CE Phosphoramidite

5'-Dabsyl-dT-CE Phosphoramidite

Glen Research

5'-Dabsyl-dT-CE Phosphoramidite

Structure

Catalog Number: 10-1532-xx

Description: 5'-Dabsyl-dT-CE Phosphoramidite

5'-Dabsyl-2'-deoxyThymidine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C33H44N7O8PS M.W.: 729.78 F.W.: 591.53

Diluent: Anhydrous Acetonitrile
Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dGwith acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2days

Dabcyl LABELLING

A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.

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5'-Dabsyl-dT-CE Phosphoramidite

5'-Dabsyl-dT-CE Phosphoramidite

Glen Research

MSDS

Glen Report 19.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Dabsyl-dT-CE Phosphoramidite

5'-Dabsyl-dT-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1532-90 100µmoles .073grams 1 20 12 7.5 5.45 4 1
10-1532-02 0.25grams .25grams 3.43 101 60.6 37.88 27.55 20.2 5.05
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1532-90 100µmoles .073grams 1.5 .07 23.6 14.75 10.73 1.48
10-1532-02 0.25grams .25grams 5.11 .07 95.8 59.88 43.55 5.99
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1532-90 100µmoles .073grams 1.5 .07 25.2 15.75 11.45

10-1532-02 0.25grams .25grams 5.11 .07 97.4 60.88 44.27

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200