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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

5'-Amino-Modifier 5

5'-Amino-Modifier 5

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1905-025'-Amino-Modifier 5 0.25g £160.00£147.20Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1905-905'-Amino-Modifier 5 100µmoles £48.00£44.16Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5'-Amino-Modifier 5

5'-Amino-Modifier 5

Glen Research

5'-Amino-Modifier 5

10-1905

Catalog Number: 10-1905-xx

Description: 5'-Amino-Modifier 5

2-[2-(4-Monomethoxytrityl)aminoethoxy]ethyl-(2-cyanoethyl)-
N,N-diisopropyl)-phosphoramidite
Formula: C33H44N3O4P M.W.: 577.71 F.W.: 167.10

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Deprotect as required by nucleobases.WARNING: As with other trityl-protected amines, drying down the oligo after cleavage and deprotection without addition of an non-volatile base (for example, TRIS) will lead to loss of the Trityl protecting group. See Technical Bulletin for details (Technical Bulletin).
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

TERMINUS MODIFIERS

Glen Research 5’-Modifiers are designed for use in DNA synthesizers to functionalize the 5’-terminus of the target oligonucleotide. The 5’-Amino-Modifiers are available with a variety of chain lengths to fit exactly the desired application.

The DMS(O)MT-protected amino group is easier to deprotect compared to the MMT-protected one. The sulfoxy derivative survives conditions of oligonucleotide synthesis and can either be cleaved with standard deblock solution, or left intact for HPLC purification. At the same time, the DMS(O)MT group is fully compatible with cartridge purification. When detritylation on a cartridge is carried out, the DMS(O)MT , which is more stable than MMT , does not reattach itself to an amine. We now offer 5’-DMS(O)MT-Amino-Modifier C6 utilizing this new trityl based protecting group.

5’-Amino-Modifier TEG, a hydrophilic triethylene glycol ethylamine derivative, is 12 atoms in length and fully soluble in aqueous media.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Amino-Modifier 5

5'-Amino-Modifier 5

Glen Research

MSDS

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Amino-Modifier 5

5'-Amino-Modifier 5

Glen Research

Technical Notes

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Amino-Modifier 5

5'-Amino-Modifier 5

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1905-02 0.25grams .25grams 4.33 131 78.6 49.13 35.73 26.2 6.55
10-1905-90 100µmoles .058grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1905-02 0.25grams .25grams 6.46 .07 122.8 76.75 55.82 7.68
10-1905-90 100µmoles .058grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1905-02 0.25grams .25grams 6.46 .07 124.4 77.75 56.55

10-1905-90 100µmoles .058grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200