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Oligo Synthesis

Oligo Synthesis : CEPs

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5,6-Dihydro-dU-CE Phosphoramidite

5,6-Dihydro-dU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1550-025,6-Dihydro-dU-CE Phosphoramidite 0.25g £476.00£437.92Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1550-905,6-Dihydro-dU-CE Phosphoramidite 100µmoles £156.00£143.52Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5,6-Dihydro-dU-CE Phosphoramidite

5,6-Dihydro-dU-CE Phosphoramidite

Glen Research

5,6-Dihydro-dU-CE Phosphoramidite

10-1550

Catalog Number: 10-1550-xx

Description: 5,6-Dihydro-dU-CE Phosphoramidite

5'-Dimethoxytrityl-5,6-dihydro-2'-deoxyUridine),3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C39H49N4O8P M.W.: 732.81 F.W.: 292.19

Diluent: Anhydrous Acetonitrile
Coupling: Monomers that allow for UltraMILD deprotection must be used. (dA:10-1601-xx,dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature or 2 hours at room temperature in Ammonium Hydroxide.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

 

DNA DAMAGE/REPAIR

Cellular DNA is constantly being damaged by oxidation and alkylation, by free radicals, and by ultraviolet and ionizing radiation. The body has therefore evolved a number of repair enzyme systems to excise and repair these lesions. The 8-oxo purine monomers allow investigation of the structure and activity of oligonucleotides containing an 8-oxo mutation which is formed naturally when DNA is subjected to oxidative conditions or ionizing radiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that are structural components of alanine transfer RNA. Dihydrouracil and the hydroxy pyrimidines are major base damage products formed by exposure of DNA to ionizing radiation.

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5,6-Dihydro-dU-CE Phosphoramidite

5,6-Dihydro-dU-CE Phosphoramidite

Glen Research

MSDS

Glen Report

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5,6-Dihydro-dU-CE Phosphoramidite

5,6-Dihydro-dU-CE Phosphoramidite

Glen Research

Oligonucleotides synthesised using 5,6-dihydro-dU or 5,6-dihydro-dT and UltraMILD monomers can be cleaved using either concentrated ammonium hydroxide or 50mM potassium carbonate in anhydrous methanol.  Complete cleavage and deprotection can be accomplished at room temperature in 2-4 hours without damaging either the dihydro-dU or dihydro-dT bases.

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5,6-Dihydro-dU-CE Phosphoramidite

5,6-Dihydro-dU-CE Phosphoramidite

Glen Research

EXTINCTION DATA

Item Nucleoside λMax-1 Emax-1 λMax-2 Emax-2 E260


(nm) (ml/µmole) nm (ml/µmole) (ml/µmole)
10-1550 dihydro-dU 210 6.3

<0.1

 

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1550-02 0.25grams .25grams 3.41 100.33 60.2 37.63 27.36 20.07 5.02
10-1550-90 100µmoles .073grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1550-02 0.25grams .25grams 5.09 .07 95.4 59.63 43.36 5.96
10-1550-90 100µmoles .073grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1550-02 0.25grams .25grams 5.09 .07 97 60.63 44.09

10-1550-90 100µmoles .073grams 1.5 .07 25.2 15.75 11.45

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