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Oligo Synthesis

Oligo Synthesis : CEPs

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3'-dG-CE Phosphoramidite

3'-dG-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1074-023'-dG-CE Phosphoramidite 0.25g £1,000.00£920.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1074-903'-dG-CE Phosphoramidite 100µmoles £360.00£331.20Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1074-953'-dG-CE Phosphoramidite 50µmoles £180.00£165.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3'-dG-CE Phosphoramidite

3'-dG-CE Phosphoramidite

Glen Research

3'-dG-CE Phosphoramidite

Structure

Catalog Number: 10-1074-xx

Description: 3'-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylformamidine-3'-deoxyGuanosine,
2'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C43H53N8O7P M.W.: 824.92 F.W.: 329.21

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

2’-5’ Linked Oligonucleotides

Cellular DNA and RNA are made up of ribo- and 2’-deoxyribonucleic acids linked together via 3’-5’ phosphodiester linkages and by far comprise the bulk of polynucleic acids found in cells. Much less common are oligonucleotides which have 2’-5’ linkages. However, a unique feature of 2’-5’ linked oligonucleotides is their ability to bind selectively to complementary RNA. These features suggest a number of interesting uses for 2’-5’ linked oligos such as their use as RNA specific probes or in antisense oligos. Recently, oligos have been synthesized using 3’-deoxy-2’-phosphoramidites and 2’-deoxy-3’-phosphoramidites to produce chimeras with 2’-5’ linked ends and 3’-5’ linked central regions. It was found that 2’-5’ phosphorothioate oligos: 1) bind selectively to complementary RNA with the same affinity as phosphodiester oligos; 2) exhibit much nonspecific binding to cellular proteins; 3) do not activate RNase H. A 3’-deoxynucleoside at the 3’-terminus of an otherwise normal oligonucleotide effectively blocks polymerase extension.

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3'-dG-CE Phosphoramidite

3'-dG-CE Phosphoramidite

Glen Research

MSDS

Glen Report 10.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3'-dG-CE Phosphoramidite

3'-dG-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1074-02 0.25grams .25grams 3.03 87.67 52.6 32.88 23.91 17.53 4.38
10-1074-90 100µmoles .082grams 1 20 12 7.5 5.45 4 1
10-1074-95 50µmoles .041grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1074-02 0.25grams .25grams 4.52 .07 84 52.5 38.18 5.25
10-1074-90 100µmoles .082grams 1.5 .07 23.6 14.75 10.73 1.48
10-1074-95 50µmoles .041grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1074-02 0.25grams .25grams 4.52 .07 85.6 53.5 38.91

10-1074-90 100µmoles .082grams 1.5 .07 25.2 15.75 11.45

10-1074-95 50µmoles .041grams .75 .07 10.2 6.38 4.64

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