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Oligo Synthesis

Oligo Synthesis : CEPs

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3-deaza-dA-CE Phosphoramidite

3-deaza-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1088-023-deaza-dA-CE Phosphoramidite 0.25g £780.00£717.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1088-903-deaza-dA-CE Phosphoramidite 100µmoles £284.00£261.28Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1088-953-deaza-dA-CE Phosphoramidite 50µmoles £142.00£130.64Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

3-deaza-dA-CE Phosphoramidite

3-deaza-dA-CE Phosphoramidite

Glen Research

3-deaza-dA-CE Phosphoramidite

Structure

Catalog Number: 10-1088-xx

Description: 3-deaza-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N-benzoyl-3-deaza-2'-deoxyAdenosine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C48H53N6O7P M.W.: 856.95 F.W.: 312.22

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Not compatible with mild deprotection at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

STRUCTURE/ACTIVITY RELATIONSHIP

The following products are used to investigate the effect on the activity of an oligonucleotide when key structural elements are changed. The 7-deaza purine monomers lack groups critical for hydrogen bonding. 7-Deaza-8-aza-A and 7-deaza-8-aza-G (PPG) monomers are isomers of A and G and have similar electron density. Their presence in oligos is slightly stabilizing relative to A and G. Unlike G, PPG does not lead to aggregation and G-rich oligos can be easily prepared and isolated. 5’-Fluorescein oligos with PPG at the 5’-terminus are much less quenched than the equivalent G oligos. As a purine analogue of Thymidine, 7-deaza-2’-deoxyXanthosine (7-deaza-dX) promises to have interesting effects on DNA structure of triplexes. 7-Deaza-dX also forms a non-standard base pair with a 2,4-diaminopyrimidine nucleoside analogue. Standard nucleobases have an unshared pair of electrons that project into the minor groove of duplex DNA. Enzymes that interact with DNA, polymerases, reverse transcriptases, restriction enzymes, etc., may use a hydrogen bond donating group to contact the hydrogen bond acceptor in the minor groove. 3-Deaza-2’-deoxyadenosine is very interesting in that it maintains the ability for regular Watson-Crick hydrogen bonding to T but is lacking the electron pair at the 3-position normally provided by N3.

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3-deaza-dA-CE Phosphoramidite

3-deaza-dA-CE Phosphoramidite

Glen Research

MSDS

Glen Report 17.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

3-deaza-dA-CE Phosphoramidite

3-deaza-dA-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1088-02 0.25grams .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-1088-90 100µmoles .086grams 1 20 12 7.5 5.45 4 1
10-1088-95 50µmoles .043grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1088-02 0.25grams .25grams 4.35 .07 80.6 50.38 36.64 5.04
10-1088-90 100µmoles .086grams 1.5 .07 23.6 14.75 10.73 1.48
10-1088-95 50µmoles .043grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1088-02 0.25grams .25grams 4.35 .07 82.2 51.38 37.36

10-1088-90 100µmoles .086grams 1.5 .07 25.2 15.75 11.45

10-1088-95 50µmoles .043grams .75 .07 10.2 6.38 4.64

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