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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-Pac-A-CE Phosphoramidite

2'-OMe-Pac-A-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3601-022'-OMe-Pac-A-CE Phosphoramidite 0.25g £50.00£46.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3601-052'-OMe-Pac-A-CE Phosphoramidite 0.5g £100.00£92.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3601-102'-OMe-Pac-A-CE Phosphoramidite 1.0g £200.00£184.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3601-902'-OMe-Pac-A-CE Phosphoramidite 100µmoles £30.00£27.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

2'-OMe-Pac-A-CE Phosphoramidite

2'-OMe-Pac-A-CE Phosphoramidite

Glen Research

2'-OMe-Pac-A-CE Phosphoramidite

Structure

Catalog Number: 10-3621-xx

Description: 2'-OMe-iPr-Pac-G-CE Phosphoramidite

5'-Dimethoxytrityl-N2-isopropylphenoxyacetyl-Guanosine,2'-O-methyl,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H62N7O10P M.W.: 976.07 F.W.: 359.24

Diluent: Anhydrous Acetonitrile
Coupling: 6 minute coupling time. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide. Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

UltraMild 2’-OMe-RNA

The use of UltraMild monomers in oligonucleotide synthesis has allowed very sensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely. The DNA and RNA monomers are currently available and we also provide this set of 2’-OMe-RNA monomers. In our version of this chemistry, we use as protecting groups phenoxyacetyl (Pac) for A, acetyl (Ac) for C, and isopropyl-phenoxyacetyl (iPr-Pac) for G.

It has become clear that acetic anhydride in the conventional capping mix can cause transamidation in situations where an amine protecting group is quite labile. This leads to acetyl protection on the amino group that may be slow to be removed. Consequently, if many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (Pac2O) in Cap A. This modification removes the possibility of exchange of the iPr-Pac protecting group on the dG with acetate from the acetic anhydride capping mix

 

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2'-OMe-Pac-A-CE Phosphoramidite

2'-OMe-Pac-A-CE Phosphoramidite

Glen Research

MSDS

Glen Report 15.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2'-OMe-Pac-A-CE Phosphoramidite

2'-OMe-Pac-A-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3621-02 0.25grams .25grams 2.56 72 43.2 27 19.64 14.4 3.6
10-3621-05 0.5grams .5grams 5.12 157.33 94.4 59 42.91 31.47 7.87
10-3621-10 1.0gram 1grams 10.25 328.33 197 123.13 89.55 65.67 16.42
10-3621-90 100µmoles .098grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3621-02 0.25grams .25grams 3.82 .07 70 43.75 31.82 4.38
10-3621-05 0.5grams .5grams 7.65 .07 146.6 91.63 66.64 9.16
10-3621-10 1.0gram 1grams 15.29 .07 299.4 187.13 136.09 18.71
10-3621-90 100µmoles .098grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3621-02 0.25grams .25grams 3.82 .07 71.6 44.75 32.55

10-3621-05 0.5grams .5grams 7.65 .07 148.2 92.63 67.36

10-3621-10 1.0gram 1grams 15.29 .07 301 188.13 136.82

10-3621-90 100µmoles .098grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200