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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-OMe-5-Br-U-CE Phosphoramidite

2'-OMe-5-Br-U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3190-022'-OMe-5-Br-U-CE Phosphoramidite 0.25g £540.00£496.80Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3190-902'-OMe-5-Br-U-CE Phosphoramidite 100µmoles £192.00£176.64Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

2'-OMe-5-Br-U-CE Phosphoramidite

2'-OMe-5-Br-U-CE Phosphoramidite

Glen Research

2'-OMe-5-Br-U-CE Phosphoramidite

Structure

Catalog Number: 10-3190-xx

Description: 2'-OMe-5-Br-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-bromo-Uridine,2'-O-methyl,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C40H48BrN4O9P M.W.: 839.72 F.W.: 399.09

Diluent: Acetonitrile:THF (1:1)
Coupling: Dissolve in Acetonitrile:THF (1:1).A 6 minute coupling time is recommended.
Deprotection: Mild deprotection: Ammonium Hyrdoxide for 24 hours at room temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

minor 2’-Ome-rna phosphoramidites

To aid in the evaluation of the structures of 2’-OMe-RNA complexes, we offer the CE phosphoramidites listed below. 2’-OMe-T is useful in triplex studies while the 2-aminopurine derivative may be tested in ribozyme studies. By supporting an additional hydrogen bond, 2,6-diaminopurine (2-amino-adenosine) binds more strongly with uridine than does adenosine. Oligonucleotides containing 2’-OMe-5-Me-C and 2’-OMe-I would be of interest to researchers involved in triplex and antisense studies using 2’-OMe-RNA. The uses of 2’-OMe-5-bromo-U phosphoramidite range from crystallographic studies due to the heavy atom to cross-linking because of its photolability. 5-Fluoro-pyrimidine nucleosides have been useful as therapeutic agents and their effect on the structure and activity of oligonucleotides may be examined using the 2’-OMe-RNA derivatives. The 2,4,6-trimethylphenyl (TMP) protected 2’-OMe-U derivative is a convertible nucleoside and reaction with ammonia leads to the 5-fluoro-dC analogue. 2’-OMe-3-deaza-5-aza-C (Reverse C) derivative has the potential to mimic in oligonucleotides 5-azacytidine, a DNA methylase inhibitor. Its ability to bind as a C will likely be diminished.

ABI-style vials are supplied unless otherwise requested (see note box).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2'-OMe-5-Br-U-CE Phosphoramidite

2'-OMe-5-Br-U-CE Phosphoramidite

Glen Research

MSDS

Glen Report 11.2

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2'-OMe-5-Br-U-CE Phosphoramidite

2'-OMe-5-Br-U-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3190-02 0.25grams .25grams 2.98 86 51.6 32.25 23.45 17.2 4.3
10-3190-90 100µmoles .084grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3190-02 0.25grams .25grams 4.44 .07 82.4 51.5 37.45 5.15
10-3190-90 100µmoles .084grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3190-02 0.25grams .25grams 4.44 .07 84 52.5 38.18

10-3190-90 100µmoles .084grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200