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Oligo Synthesis

Oligo Synthesis : CEPs

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2-F-dI-CE Phosphoramidite

2-F-dI-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1082-022-F-dI-CE Phosphoramidite 0.25g £780.00£741.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1082-902-F-dI-CE Phosphoramidite 100µmoles £288.00£273.60Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1082-952-F-dI-CE Phosphoramidite 50µmoles £144.00£136.80Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

2-F-dI-CE Phosphoramidite

2-F-dI-CE Phosphoramidite

Glen Research

2-F-dI-CE Phosphoramidite

Structure

Catalog Number: 10-1082-xx

Description: 2-F-dI-CE Phosphoramidite

5'-Dimethoxytrityl-2-fluoro-O6-p-nitrophenylethyl-2'-deoxyInosine,3'-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C48H53FN7O9P M.W.: 921.96 F.W.: varies, 2F=332.18

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_2-F-dI.pdf). Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

CONVERTIBLE NUCLEOSIDES

The convertible nucleoside strategy is one of the most versatile methods for producing modifications in bases to examine their effects on DNA structure and activity. In some cases, with versatility comes difficulty in that the convertible base is modified after oligonucleotide synthesis. The chemistry is sometimes complex and base composition analysis of the final oligonucleotide is required to verify structure. The convertible dU monomer can be used to introduce a variety of modifications at the convertible position, including N, O and S modifications. Convertible F-dC is by far the simplest approach to the preparation of oligonucleotides containing F-dC - normal ammonium hydroxide treatment effects the conversion to F-dC. Convertible dA has been used to prepare oligonucleotides containing multiple points for attachment to solid supports. In this way, high capacity affinity supports for the purification of DNA binding proteins have been prepared. 2-F-dI is a convertible nucleoside for the preparation of 2’-dG derivatives following the displacement of the 2-fluorine by primary amines.

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2-F-dI-CE Phosphoramidite

2-F-dI-CE Phosphoramidite

Glen Research

MSDS

Glen Report 15.1

Glen Report 16.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2-F-dI-CE Phosphoramidite

2-F-dI-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1082-02 0.25grams .25grams 2.71 77 46.2 28.88 21 15.4 3.85
10-1082-90 100µmoles .092grams 1 20 12 7.5 5.45 4 1
10-1082-95 50µmoles .046grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1082-02 0.25grams .25grams 4.05 .07 74.6 46.63 33.91 4.66
10-1082-90 100µmoles .092grams 1.5 .07 23.6 14.75 10.73 1.48
10-1082-95 50µmoles .046grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1082-02 0.25grams .25grams 4.05 .07 76.2 47.63 34.64

10-1082-90 100µmoles .092grams 1.5 .07 25.2 15.75 11.45

10-1082-95 50µmoles .046grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200