Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

GalNAc C3 Phosphoramidite

Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65°C, 17 hours at room temperature, AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65 °C and UltraMild deprotection conditions. Note extended deprotection in ammonium hydroxide (17 hours at 55 °C) will lead to some degradation.

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1974-02GalNAc C3 Phosphoramidite 0.25g £400.00£380.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1974-90GalNAc C3 Phosphoramidite 100µmoles £204.00£193.80Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1974-95GalNAc C3 Phosphoramidite 50µmoles £110.00£104.50Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-01GalNAc C3 CPG0.1g £50.00£47.50Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-10GalNAc C3 CPG1.0g £256.00£243.20Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-13GalNAc C3 CPG1x10 µmole £144.00£136.80Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-14GalNAc C3 CPG1x 15 µmole £224.00£212.80Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-41GalNAc C3 CPG4x 1.0 µmoles £80.00£76.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
20-2974-42GalNAc C3 CPG4x0.2 µmole £60.00£57.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

GalNAc C3 Phosphoramidite

Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65°C, 17 hours at room temperature, AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65 °C and UltraMild deprotection conditions. Note extended deprotection in ammonium hydroxide (17 hours at 55 °C) will lead to some degradation.

Glen Research

N-Acetylgalactosamine (GalNAc) Oligonucleotide Conjugates

A directed approach to the delivery of therapeutic oligonucleotides specifically to the liver has been to target the asialoglycoprotein receptor (ASGPR) using a suitable glycoconjugate. Indeed, ASGPR is the ideal target for delivery of therapeutic oligonucleotides to the liver since it combines tissue specificity, high expression levels and rapid internalization and turnover. The use of oligonucleotide glycoconjugates has led to significant advances in therapeutic delivery as evidenced by the work of Alnylam Pharmaceuticals and Ionis Pharmaceuticals using multivalent N-acetylgalactosamine (GalNAc) oligonucleotide conjugates.

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. We have demonstrated that 5'-GalNAc C3 phosphoramidite can be used to prepare oligonucleotides with multiple consecutive GalNAc additions at the 5' terminus.
Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC.

 

Diluent: Anhydrous Acetonitrile

Coupling: 12 minute coupling time is recommended.

Deprotection: As required by nucleobases. Compatible with 30% ammonium hydroxide for 2 hours at 65°C, 17 hours at room temperature, AMA (ammonium hydroxide/40% methylamine 1:1) for 10 minutes at 65 °C and UltraMild deprotection conditions. Note extended deprotection in ammonium hydroxide (17 hours at 55 °C) will lead to some degradation.

Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 Days

Please Note: GalNAc C3 products are sold under agreement with AM Chemicals LLC.

 

 

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