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Oligo Synthesis

Oligo Synthesis : CEPs

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Pac-2-Amino-dA-CE Phosphoramidite

Pac-2-Amino-dA-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1585-02Pac-2-Amino-dA-CE Phosphoramidite0.25g £200.00£190.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1585-90Pac-2-Amino-dA-CE Phosphoramidite100umole £100.00£95.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1585-95Pac-2-Amino-dA-CE Phosphoramidite50umole £56.00£53.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Pac-2-Amino-dA-CE Phosphoramidite

Pac-2-Amino-dA-CE Phosphoramidite

Glen Research

Pac-2-Amino-dA-CE Phosphoramidite

5'-Dimethoxytrityl-N2-phenoxyacetyl-N6-di-n-butylaminomethylidene-2,6-diamino-2'-deoxypurine riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C57H72N9O8
M.W.: 1042.21 
F.W.: 328.22
 
The simplest approach to the design of high affinity primers and probes is to substitute A sites with 2-amino-A, since the 2-amino-A-T base pair is equivalent in strength to the G-T base pair. 2-Amino-A also destabilizes A-G wobble mismatches, thus increasing specificity. In 1998, we introduced a 2-amino-dA monomer which exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. We now recommend the use of 0.5 M CSO in anhydrous acetonitrile (40-4632-xx) for best results with multiple additions of 2-amino-dA. This is because the bis formamidine protected 2-amino-dA leads to significant strand scission when standard iodine oxidation is used during synthesis. For this reason, we have also added Pac-2-Amino-dA, a monomer with optimized protection to meet the following criteria: stable during oligonucleotide synthesis, oxidation, and detritylation; labile towards common deprotection conditions (NH3, AMA, MeNH2); and the nucleobase protecting groups are cleaved under fairly mild conditions.

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Pac-2-Amino-dA-CE Phosphoramidite

Pac-2-Amino-dA-CE Phosphoramidite

Glen Research

Glen Report 27.2: New Product - Pac-2'-Amino-dA

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Pac-2-Amino-dA-CE Phosphoramidite

Pac-2-Amino-dA-CE Phosphoramidite

Glen Research

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: 30% Ammonium Hydroxide for 4 hours at 65 °C or 8-17 hours at 55 °C. Compatible with 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) for 20 minutes at 65 °C.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 days

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200