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Oligo Synthesis

Oligo Synthesis : CEPs

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1-Ethynyl-dSpacer CE Phosphoramidite

1-Ethynyl-dSpacer CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1910-021-Ethynyl-dSpacer CE Phosphoramidite0.25g £1,000.00£920.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1910-901-Ethynyl-dSpacer CE Phosphoramidite100umole £272.00£250.24Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1910-951-Ethynyl-dSpacer CE Phosphoramidite50umole £144.00£132.48Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

1-Ethynyl-dSpacer CE Phosphoramidite

1-Ethynyl-dSpacer CE Phosphoramidite

Glen Research

1-Ethynyl-dSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1'-ethynyl-2'-deoxyribose-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Formula: C37H45N2O6P
M.W.: 644.74
F.W.: 204.12

1-Ethynyl-dSpacer CE Phosphoramidite can be used in any position within an oligonucleotide while still retaining the high efficiency of click chemistry. The modifier is efficiently incorporated into oligonucleotides using standard phosphoramidite chemistry, is stable to common deprotection conditions, and is compatible with Glen-Pak™ purification. 1-Ethynyl-dSpacer generates a substituted 1,2,3-triazole pseudo-nucleobase after click chemistry conjugation with an azide The 1-ethynyl-dSpacer modification exhibits similar duplex stability to the standard dSpacer (10-1914) and destabilizes the duplex when internally incorporated. Upon cycloaddition, the duplex stability is moderated by the resulting structure of the modification. Simple 1,2,3-triazoles were destabilizing, as were modifications that incorporated TEG linkers (6-FAM-TEG and Amino-TEG). Modifications that incorporated aromatic functional groups restored duplex stability to varying degrees with coumarin and psoralen significantly restoring stability.

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1-Ethynyl-dSpacer CE Phosphoramidite

1-Ethynyl-dSpacer CE Phosphoramidite

Glen Research

Glen Report 27.2: New Product - 1-Ethynyl-dSpacer-CE Phosphoramidite

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1-Ethynyl-dSpacer CE Phosphoramidite

1-Ethynyl-dSpacer CE Phosphoramidite

Glen Research

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Compatible with all standard deprotection conditions. Best results are obtained with AMA, 65°C, 10 minutes.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 days

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200