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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

5'-Amino-Modifier TEG PDA

5'-Amino-Modifier TEG PDA

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1949-025'-Amino-Modifier TEG PDA0.25g £336.00£319.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1949-905'-Amino-Modifier TEG PDA100 µmole £84.00£79.80Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

5'-Amino-Modifier TEG PDA

5'-Amino-Modifier TEG PDA

Glen Research

5'-Amino-Modifier TEG PDA

Structure

Catalog Number: 10-1949-xx

Description: 5'-Amino-Modifier TEG PDA

10-(N2-methylphthalimidyl-N1-ethyl)-triethyleneglycol-
1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C26H43N4O7P M.W.: 554.62 F.W.: 255.21

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Our more recent 5’-amino modifiers, protected by a novel phthalic acid diamide (PDA) protecting group, are stable solids . In contrast to the TFA protected amino modifiers, which are viscous oils, the analogous PDA protected compounds are granular powders. This important property of these compounds allows straightforward handling, storage and aliquoting and leads to a significant increase in stability.

Deprotection with methylamine in gas phase or aqueous solution or AMA leads to fast and complete removal of the PDA protecting group. However, ammonium hydroxide will not drive the equilibrium reaction to completion and only partial deprotection occurs - overnight deprotection with ammonium hydroxide will yield around 80% active amine.

We are offering three PDA Amino-Modifiers:

  •  
    • 5’-Amino-Modifier C6-PDA

    • Hydrophobic 5’-Amino-Modifier C12-PDA

    • Hydrophilic 5’-Amino-Modifier-TEG-PDA

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Amino-Modifier TEG PDA

5'-Amino-Modifier TEG PDA

Glen Research

Material Safety Data Sheet

Glen Report 24.1: New Products - Solid, Stable amino-Modifiers - PDA amino-Modifiers

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Amino-Modifier TEG PDA

5'-Amino-Modifier TEG PDA

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1949-90 100µmoles .055grams 1 20 12 7.5 5.45 4 1
10-1949-02 0.25grams .25grams 4.51 137 82.2 51.38 37.36 27.4 6.85
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1949-90 100µmoles .055grams 1.5 .07 23.6 14.75 10.73 1.48
10-1949-02 0.25grams .25grams 6.73 .07 128.2 80.13 58.27 8.01
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1949-90 100µmoles .055grams 1.5 .07 25.2 15.75 11.45

10-1949-02 0.25grams .25grams 6.73 .07 129.8 81.13 59

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200