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Oligo Synthesis

Oligo Synthesis : CEPs

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S-Bz-Thiol-Modifier C6-dT

S-Bz-Thiol-Modifier C6-dT

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1538-02S-Bz-Thiol-Modifier C6-dT0.25g £440.00£404.80Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1538-90S-Bz-Thiol-Modifier C6-dT100 µmole £196.00£180.32Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1538-95S-Bz-Thiol-Modifier C6-dT50 µmole £104.00£95.68Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

S-Bz-Thiol-Modifier C6-dT

S-Bz-Thiol-Modifier C6-dT

Glen Research

S-Bz-Thiol-Modifier C6-dT

Structure

Catalog Number: 10-1538-xx

Description: S-Bz-Thiol-Modifier C6-dT

5'-(4,4'-Dimethoxytrityl)-5-[N-(6-(3-benzoylthiopropanoyl)-
aminohexyl)- 3-acrylamido]-2'-deoxyuridine, 3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C58H71N6O11PS M.W.: 1091.26 F.W.: 546.53

Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended.
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx). Prior to cleavage, treat support with 10% diethylamine in ACN for 5 minutes at room temperature and rinse with ACN to remove the cyanoethyl protecting groups. Then cleave and deprotect the oligo using 30% ammonium hydroxide/40% methylamine 1:1 (AMA) for 2 hours at room temperature. Prior to use, reduce any disulfide formation using 100 mM TCEP or DTT for 30 minutes at room temperature.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 1-2 days

Our repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the Carboxy-Modifier C10, it is possible to label one or multiple sites within an oligonucleotide. This opens up the possibility to label any number of different dyes or molecules within an oligonucleotide when the phosphoramidite is unavailable. Doing so is straightforward and may be done manually off the synthesizer or even in a fully-automated manner on the DNA synthesizer.

We have never found conditions which allow the TFA group to be removed from an amino-modifier while the oligonucleotide remains attached to the support. We are able to solve this problem by using a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group. The Fmoc group is removed using a two step procedure, the first to remove the cyanoethyl protection groups and flush the formed acrylonitrile from the synthesis column using 1% diisopropylamine in acetonitrile, and the second to remove the Fmoc group using 10% piperidine in DMF. The amino group so formed on the column can be reacted with a variety of activated esters. We offer Fmoc-Amino-Modifier C6 dT Phosphoramidite as a nucleosidic option and Amino-Modifier Serinol Phosphoramidite as a non-nucleosidic alternative. We also offer S-Bz-Thiol-Modifier C6-dT to join the ranks of thiol-modifiers for oligonucleotide synthesis. Thiol-Modifier C6-dT can be added as usual at the desired locations within a sequence.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

S-Bz-Thiol-Modifier C6-dT

S-Bz-Thiol-Modifier C6-dT

Glen Research

Material Safety Data Sheet

??Glen Report 24.1: New Product - S-Bz-Thiol-Modifier C6-dT

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

S-Bz-Thiol-Modifier C6-dT

S-Bz-Thiol-Modifier C6-dT

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1538-90 100µmoles .109grams 1 20 12 7.5 5.45 4 1
10-1538-02 0.25grams .25grams 2.29 63 37.8 23.63 17.18 12.6 3.15
10-1538-95 50µmoles .055grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1538-90 100µmoles .109grams 1.5 .07 23.6 14.75 10.73 1.48
10-1538-02 0.25grams .25grams 3.42 .07 62 38.75 28.18 3.88
10-1538-95 50µmoles .055grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1538-90 100µmoles .109grams 1.5 .07 25.2 15.75 11.45

10-1538-02 0.25grams .25grams 3.42 .07 63.6 39.75 28.91

10-1538-95 50µmoles .055grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200