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Oligo Synthesis

Oligo Synthesis : CEPs

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tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1518-02tCnitro-CE Phosphoramidite0.25g £1,168.00£1,074.56Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1518-90tCnitro-CE Phosphoramidite100umole £416.00£382.72Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1518-95tCnitro-CE Phosphoramidite50umole £212.00£195.04Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

 

structure 10-1518

Catalog Number: 10-1518-xx

Description: tCnitro-CE Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-1'-(7-nitro-1,3-diaza-2-oxophenothiazin-1-yl)-
2'-deoxy-Β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C45H49N6O9PS M.W.: 880.94 F.W.: 440.32

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure. This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine.

 

 

 



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tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

Glen Report 22.1: tC, tCo and tCnitro: Tricyclic Cytosine Probes of DNA and RNA Structure

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

Absorption and emisssion data for tC and tCo are collected below:

 

tC
QY
l
e
(L/mol.cm)
single-stranded
0.21
385nm
 

double-stranded
0.19
 

 

tCo
QY
l
e
(L/mol.cm)
single-stranded
0.30
360nm
9000
double-stranded
0.21


(QY determined relative to quinine sulfate in 0.5M H2SO4)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

tCnitro-CE Phosphoramidite

tCnitro-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1518-95 50µmoles .044grams .5 3.33 2 1.25 .91 .67 .17
10-1518-90 100µmoles .088grams 1 20 12 7.5 5.45 4 1
10-1518-02 0.25grams .25grams 2.84 81.33 48.8 30.5 22.18 16.27 4.07
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1518-95 50µmoles .044grams .75 .07 8.6 5.38 3.91 .54
10-1518-90 100µmoles .088grams 1.5 .07 23.6 14.75 10.73 1.48
10-1518-02 0.25grams .25grams 4.24 .07 78.4 49 35.64 4.9
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1518-95 50µmoles .044grams .75 .07 10.2 6.38 4.64

10-1518-90 100µmoles .088grams 1.5 .07 25.2 15.75 11.45

10-1518-02 0.25grams .25grams 4.24 .07 80 50 36.36

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