Catalog Number: 10-4410-xx
Description: UniCap Phosphoramidite
|Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
|Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.
|Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
|Storage: Freezer storage, -10 to -30°C, dry
|Stability in Solution: 7 days
|Please Note: This product is supplied under license.
Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution.
ALTERNATIVE CAPPING REAGENT
The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.
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