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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

UniCap Phosphoramidite

UniCap Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-4410-02UniCap Phosphoramidite 0.25g £40.00£38.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-4410-05UniCap Phosphoramidite 0.5g £80.00£76.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-4410-10UniCap Phosphoramidite 1.0g £160.00£152.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-4410-20UniCap Phosphoramidite 2.0g £320.00£304.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

UniCap Phosphoramidite

UniCap Phosphoramidite

Glen Research

UniCap Phosphoramidite

STrucutre

Catalog Number: 10-4410-xx

Description: UniCap Phosphoramidite

Diethyleneglycol ethyl ether (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C15H31N2O4P M.W.: 334.39

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 7 days
Please Note: This product is supplied under license.

ALTERNATIVE SOLVENTS/REAGENTS

Glen Research offers alternative solvents and reagents in suitable bottles and formulations for use on various DNA synthesizers. All solvents and reagents are prepared to our exacting specifications to ensure the highest coupling efficiencies and are passed through a 0.2 micron filter during packaging to eliminate particulate contamination. Glen Research offers the activators below in powder form for later dissolution in anhydrous acetonitrile or as a prepared solution.

ALTERNATIVE CAPPING REAGENT

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent. To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument. Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step. The standard capping steps can be left out of the cycle. UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

UniCap Phosphoramidite

UniCap Phosphoramidite

Glen Research

Material Safety Data Sheet

??Glen Report 17.1: UniCap Phosphoramidite, An alternative to acetic anhydride capping

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

UniCap Phosphoramidite

UniCap Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-4410-02 0.25grams .25grams 7.48 236 141.6 88.5 64.36 47.2 11.8
10-4410-05 0.5grams .5grams 14.95 485 291 181.88 132.27 97 24.25
10-4410-10 1.0gram 1grams 29.91 983.67 590.2 368.88 268.27 196.73 49.18
10-4410-20 2.0grams 2grams 59.81 1980.33 1188.2 742.63 540.09 396.07 99.02
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-4410-02 0.25grams .25grams 11.16 .07 216.8 135.5 98.55 13.55
10-4410-05 0.5grams .5grams 22.32 .07 440 275 200 27.5
10-4410-10 1.0gram 1grams 44.63 .07 886.2 553.88 402.82 55.39
10-4410-20 2.0grams 2grams 89.27 .07 1779 1111.88 808.64 111.19
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-4410-02 0.25grams .25grams 11.16 .07 218.4 136.5 99.27

10-4410-05 0.5grams .5grams 22.32 .07 441.6 276 200.73

10-4410-10 1.0gram 1grams 44.63 .07 887.8 554.88 403.55

10-4410-20 2.0grams 2grams 89.27 .07 1780.6 1112.88 809.36

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200