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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

TAMRA-dT

TAMRA-dT

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1057-02TAMRA-dT 0.25g £780.00£741.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1057-90TAMRA-dT 100µmoles £396.00£376.20Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1057-95TAMRA-dT 50µmoles £200.00£190.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

TAMRA-dT

TAMRA-dT

Glen Research

TAMRA-dT

Structure

Catalog Number: 10-1057-xx

Description: TAMRA-dT

5'-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-
3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C73H83N8O13P M.W.: 1311.48 F.W.: 870.85

Diluent: 10% THF in Anhydrous Acetonitrile
Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution.A 6 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx)
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

RHODAMINE LABELLING

Rhodamine derivatives are not sufficiently stable to survive conventional deprotection and these must be attached to amino-modified oligonucleotides using post-synthesis labelling techniques. Because Tetramethyl Rhodamine (TAMRA) is not base stable, the procedure to cleave and deprotect the labelled oligonucleotide must be carefully considered. Using the UltraMILD monomers and deprotection with potassium carbonate in methanol, TAMRA oligonucleotides can be fairly conveniently isolated. To streamline the preparation of TAMRA oligos, we offer 3’-TAMRA CPG for 3’ labelling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TAMRA-dT

TAMRA-dT

Glen Research

TAMRA-dT

TAMRA-dT

Glen Research

5'-Dimethoxytrityloxy-5-[N-((tetramethylrhodaminyl)-aminohexyl)-
3-acrylimido]-2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C73H83N8O13P M.W.: 1311.48 F.W.: 870.85

Diluent: 10% THF in Anhydrous Acetonitrile
Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). Allow 15 minutes for the product to go into solution.A 6 minute coupling time is recommended. Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx.). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx)
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours
  Material Safety Data Sheet

Literature Highlights

Glen Report 11.1: NEW FLUORESCENT REAGENTS - TAMRA-dT, DABCYL-dT
Glen Report 21.2: Deprotection - Volume 3 - Dye-Containing Oligonucleotides

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1057-02 0.25grams .25grams 1.91 50.33 30.2 18.88 13.73 10.07 2.52
10-1057-90 100µmoles .131grams 1 20 12 7.5 5.45 4 1
10-1057-95 50µmoles .066grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1057-02 0.25grams .25grams 2.85 .067 50.6 31.63 23 3.16  
10-1057-90 100µmoles .131grams 1.5 .067 23.6 14.75 10.73 1.48  
10-1057-95 50µmoles .066grams .75 .067 8.6 5.38 3.91 .54  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1057-02 0.25grams .25grams 2.85 .067 52.2 32.63 23.73    
10-1057-90 100µmoles .131grams 1.5 .067 25.2 15.75 11.45    
10-1057-95 50µmoles .066grams .75 .067 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TAMRA-dT

TAMRA-dT

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1057-02 0.25grams .25grams 1.91 50.33 30.2 18.88 13.73 10.07 2.52
10-1057-90 100µmoles .131grams 1 20 12 7.5 5.45 4 1
10-1057-95 50µmoles .066grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1057-02 0.25grams .25grams 2.85 .07 50.6 31.63 23 3.16
10-1057-90 100µmoles .131grams 1.5 .07 23.6 14.75 10.73 1.48
10-1057-95 50µmoles .066grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1057-02 0.25grams .25grams 2.85 .07 52.2 32.63 23.73

10-1057-90 100µmoles .131grams 1.5 .07 25.2 15.75 11.45

10-1057-95 50µmoles .066grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200