Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Symmetric Doubler Phosphoramidite

Symmetric Doubler Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1920-02Symmetric Doubler Phosphoramidite 0.25g £192.00£182.40Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1920-90Symmetric Doubler Phosphoramidite 100µmoles £120.00£114.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Symmetric Doubler Phosphoramidite

Symmetric Doubler Phosphoramidite

Glen Research

Symmetric Doubler Phosphoramidite

STructure

Catalog Number: 10-1920-xx

Description: Symmetric Doubler Phosphoramidite

1,3-bis-[5-(4,4'-dimethoxytrityloxy)pentylamido]propyl-2-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C64H79N4O10P M.W.: 1095.32 F.W.: 351.31

Diluent: Anhydrous Acetonitrile
Coupling: Synthesis should be carried out on 1000? support. A 15 minute coupling time is recommended for the doubler addition.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days
Please Note: This product is supplied under license from ISIS Innovation Limited.

DENDRIMERS

Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label usingγ-32P-ATP and polynucleotide kinase increases in proportion to the number of 5'-ends. Fluorescent signal also increases in proportion to the number of 5'-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the ‘bottom up' approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Symmetric Doubler Phosphoramidite

Symmetric Doubler Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 12.1: OLIGONUCLEOTIDE DENDRIMERS: FROM POLY-LABELLED DNA PROBES TO STABLE NANO-STRUCTURES

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Symmetric Doubler Phosphoramidite

Symmetric Doubler Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1920-02 0.25grams .25grams 2.28 62.67 37.6 23.5 17.09 12.53 3.13
10-1920-90 100µmoles .11grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1920-02 0.25grams .25grams 3.41 .07 61.8 38.63 28.09 3.86
10-1920-90 100µmoles .11grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1920-02 0.25grams .25grams 3.41 .07 63.4 39.63 28.82

10-1920-90 100µmoles .11grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200