Catalog Number: 40-4037-xx
Description: Sulfurizing Reagent II
Diluent: 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
*For a 0.05M solution:
Fully dissolve 1g in 60mL pyridine, and dilute with 40mL acetonitrile,
Fully dissolve 2g in 120mL pyridine, and dilute with 80mL acetonitrile
See technical bulletin for details.
|Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
|Deprotection: See TB_Sulfurizing II.pdf Technical Bulletin
|Storage: Controlled room temperature or lower, dry
|Stability in Solution: At least 6 months
Glen Research’s Sulfurizing Reagents are used to prepare phosphorothioate linkages using CE phosphoramidite chemistry. Each reagent exhibits the following attributes:
1) Reliably soluble, making them safe to use on automated synthesizers.
2) Reaction is fast (30 seconds), making the process convenient on small scales and readily amenable to scale-up.
3) Process is efficient, with better than 96% of the linkages being phosphorothioate and the remainder being phosphodiester.
When ordering Sulfurizing Reagent (Beaucage Reagent), please also order the silanized bottle appropriate for your instrument. There will be no charge for one bottle when supplied with a reagent order. The silanized bottle can be reused after rinsing with acetonitrile and drying. Additional silanized bottles are priced as shown below. Dissolve the reagent in acetonitrile at a concentration of 1g/100mL.
Sulfurizing Reagent II (3-((Dimethylamino-methylidene)amino)-3H-1,2,4-dithiazole-3-thione, DDTT) exhibits all the properties of Beaucage Reagent while adding stability in solution on the synthesizer AND offering strong ability to sulfurize RNA linkages. Sulfurizing Reagent II is available in powder form and as a stable solution.
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