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Oligo Synthesis

Oligo Synthesis : Reagents

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For multiples of the same product please request a quote.

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Sulfurizing Reagent II

Sulfurizing Reagent II

Glen Research

Catalogue No.DescriptionPack SizePriceQty
40-4037-10Sulfurizing Reagent II 1.0g £40.00£36.80Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
40-4037-20Sulfurizing Reagent II 2.0g £80.00£73.60Offer until : 31-Dec-2019Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Sulfurizing Reagent II

Sulfurizing Reagent II

Glen Research

Sulfurizing Reagent II

Structure

Catalog Number: 40-4037-xx

Description: Sulfurizing Reagent II

3-((N,N-dimethylaminomethylidene)amino)-3H-1,2,4-dithiazole-5-thione
Formula: C5H7N3S3 M.W.: 205.31

Diluent: 60% pyridine/40% Acetonitrile (fully dissolve in pyridine first, see notes below*)
*For a 0.05M solution:
Fully dissolve 1g in 60mL pyridine, and dilute with 40mL acetonitrile,
Fully dissolve 2g in 120mL pyridine, and dilute with 80mL acetonitrile
See technical bulletin for details.
Sulfurizing: A 60 second sulfurization step time for DNA or a 360 second sulfurization step time for RNA is recommended. Sulfurization step must come before capping step to minimize phosphodiester formation.
Deprotection: See TB_Sulfurizing II.pdf Technical Bulletin
Storage: Controlled room temperature or lower, dry
Stability in Solution: At least 6 months

SULFURIZING REAGENT

Glen Research’s Sulfurizing Reagents are used to prepare phosphorothioate linkages using CE phosphoramidite chemistry. Each reagent exhibits the following attributes:
1) Reliably soluble, making them safe to use on automated synthesizers.
2) Reaction is fast (30 seconds), making the process convenient on small scales and readily amenable to scale-up.
3) Process is efficient, with better than 96% of the linkages being phosphorothioate and the remainder being phosphodiester.
When ordering Sulfurizing Reagent (Beaucage Reagent), please also order the silanized bottle appropriate for your instrument. There will be no charge for one bottle when supplied with a reagent order. The silanized bottle can be reused after rinsing with acetonitrile and drying. Additional silanized bottles are priced as shown below. Dissolve the reagent in acetonitrile at a concentration of 1g/100mL.

Sulfurizing Reagent II (3-((Dimethylamino-methylidene)amino)-3H-1,2,4-dithiazole-3-thione, DDTT) exhibits all the properties of Beaucage Reagent while adding stability in solution on the synthesizer AND offering strong ability to sulfurize RNA linkages. Sulfurizing Reagent II is available in powder form and as a stable solution.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Sulfurizing Reagent II

Sulfurizing Reagent II

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
.05M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
40-4037-10 1gram 1grams N/A N/A N/A N/A N/A N/A N/A
40-4037-20 2grams 2grams N/A N/A N/A N/A N/A N/A N/A
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
40-4037-10 1gram 1grams N/A N/A N/A N/A N/A
40-4037-20 2grams 2grams N/A N/A N/A N/A N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
40-4037-10 1gram 1grams N/A N/A N/A N/A
40-4037-20 2grams 2grams N/A N/A N/A N/A

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200