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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

SIMA (HEX) Phosphoramidite

SIMA (HEX) Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-5905-02SIMA (HEX) Phosphoramidite 0.25g £320.00£304.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-5905-90SIMA (HEX) Phosphoramidite 100µmoles £140.00£133.00Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-5905-95SIMA (HEX) Phosphoramidite 50µmoles £72.00£68.40Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

SIMA (HEX) Phosphoramidite

SIMA (HEX) Phosphoramidite

Glen Research

SIMA (HEX) Phosphoramidite

Structure

Catalog Number: 10-5905-xx

Description: SIMA (HEX) Phosphoramidite

6-(4,7-Dichloro-2',7'-diphenyl-3',6'-dipivaloylfluorescein-6-carboxamido)-
hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C58H64N3O10Cl2P M.W.: 1065.02 F.W.: 759.54

Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days, <90% efficient after 4 days

FLUORESCEIN LABELLING

5’-Fluorescein phosphoramidite contains no 4,4’-dimethoxytrityl (DMT) group and can be added only once at the 5’-terminus, thereby terminating synthesis. This product is prepared using the 6-carboxyfluorescein derivative. The tetrachloro-, hexachloro-and dichloro-dimethoxy-fluorescein phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers. Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling. The analogous structure, 6-Fluorescein Phosphoramidite, prepared using 6-FAM, is also available, along with 6-Fluorescein Serinol Phosphoramidite. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.

We offer five fluorescein supports. Fluorescein CPG has traditionally been used to add the fluorescein label at the 3’-terminus. The analogous structure, 3’-(6-Fluorescein) CPG, prepared using 6-FAM, is now also available, along with 6-Fluorescein Serinol CPG. We also offer 3’-(6-FAM) CPG and Fluorescein-dT CPG, both derivatives of 6-carboxyfluorescein (6-FAM). Both are single isomers and use an amide linkage which is stable during cleavage and deprotection and does not allow isomer formation. 3’-(6-FAM) CPG allows effective blockage of the 3’-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generates the fluorescein labelled oligos.

FLUORESCEIN LAELLING (SIMA)

Dichloro-diphenyl-fluorescein, SIMA (HEX) exhibits virtually identical absorbance and emission spectra to HEX. SIMA (HEX) is much more stable to basic deprotection conditions than HEX and oligonucleotides can be deprotected using ammonium hydroxide at elevated temperatures and even ammonium hydroxide/methylamine (AMA) at room temperature or 65°C for 10 minutes. SIMA absorption maximum was 3 nm blue-shifted compared to HEX at pH 7. The absorbance is broader, so the extinction coefficient is smaller than that of HEX, but when exciting at 500 nm where the absorbance was normalized, the emission was still 90% of HEX and the emission was red-shifted by 5 nm. A second SIMA (HEX) product, SIMA (HEX)-dT, can be used to introduce SIMA (HEX) in the synthetic oligonucleotide sequence, usually as a replacement for the native dT linkage. Again, this product is fully compatible with deprotection schemes using ammonium hydroxide at elevated temperatures or AMA at room temperature and 65°C.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

SIMA (HEX) Phosphoramidite

SIMA (HEX) Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 21.1: NEW FLUORESCENT PHOSPHORAMIDITES - SIMA (HEX), DYLIGHT™

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

SIMA (HEX) Phosphoramidite

SIMA (HEX) Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-5905-95 50µmoles .053grams .5 3.33 2 1.25 .91 .67 .17
10-5905-90 100µmoles .107grams 1 20 12 7.5 5.45 4 1
10-5905-02 0.25grams .25grams 2.35 65 39 24.38 17.73 13 3.25
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-5905-95 50µmoles .053grams .75 .07 8.6 5.38 3.91 .54
10-5905-90 100µmoles .107grams 1.5 .07 23.6 14.75 10.73 1.48
10-5905-02 0.25grams .25grams 3.5 .07 63.6 39.75 28.91 3.98
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-5905-95 50µmoles .053grams .75 .07 10.2 6.38 4.64

10-5905-90 100µmoles .107grams 1.5 .07 25.2 15.75 11.45

10-5905-02 0.25grams .25grams 3.5 .07 65.2 40.75 29.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200