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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

rSpacer CE Phosphoramidite

rSpacer CE Phosphoramidite

Glen Research

rSpacer CE Phosphoramidite

rSpacer CE Phosphoramidite

Glen Research

rSpacer CE Phosphoramidite

Structure

Catalog Number: 10-3914-xx

Description: rSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1'-Deoxyribose-2'-O-Triisopropylsilyloxymethyl-
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C45H67N2O8PSi M.W.: 823.09 F.W.: 196.09

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: 6 minute coupling recommended.
Deprotection: As required by ribonucleobases. See Technical Bulletin for details (Technical Bulletin).
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

rSpacer is used to introduce an abasic site to an RNA sequence.

The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

rSpacer CE Phosphoramidite

rSpacer CE Phosphoramidite

Glen Research

rSpacer CE Phosphoramidite

rSpacer CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3914-02 0.25grams .25grams 3.04 88 52.8 33 24 17.6 4.4
10-3914-90 100µmoles .082grams 1 20 12 7.5 5.45 4 1
10-3914-95 50µmoles .041grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-3914-02 0.25grams .25grams 4.53 .07 84.2 52.63 38.27 5.26  
10-3914-90 100µmoles .082grams 1.5 .07 23.6 14.75 10.73 1.48  
10-3914-95 50µmoles .041grams .75 .07 8.6 5.38 3.91 .54  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-3914-02 0.25grams .25grams 4.53 .07 85.8 53.63 39    
10-3914-90 100µmoles .082grams 1.5 .07 25.2 15.75 11.45    
10-3914-95 50µmoles .041grams .75 .07 10.2 6.38 4.64  

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200