|rSpacer CE Phosphoramidite
Catalog Number: 10-3914-xx
Description: rSpacer CE Phosphoramidite
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
|Coupling: 6 minute coupling recommended.
|Deprotection: As required by ribonucleobases. See Technical Bulletin for details (Technical Bulletin).
|Storage: Freezer storage, -10 to -30°C, dry
|Stability in Solution: 2-3 days
MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)
Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.
Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.
rSpacer is used to introduce an abasic site to an RNA sequence.
The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.
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