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Oligo Synthesis

Oligo Synthesis : CEPs

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Pyrrolidine-CE Phosphoramidite

Pyrrolidine-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1915-02Pyrrolidine-CE Phosphoramidite 0.25g £868.00£824.60Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1915-90Pyrrolidine-CE Phosphoramidite 100µmoles £304.00£288.80Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1915-95Pyrrolidine-CE Phosphoramidite 50µmoles £152.00£144.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Pyrrolidine-CE Phosphoramidite

Pyrrolidine-CE Phosphoramidite

Glen Research

Pyrrolidine-CE Phosphoramidite

Structure

Catalog Number: 10-1915-xx

Description: Pyrrolidine-CE Phosphoramidite

(2R, 3S)-2-Dimethoxytrityloxymethyl-N-(fluorenylmethyl)-pyrrolidin-
3-yl-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C50H56N3O7P M.W.: 841.97 F.W.: 178.10

Diluent: Anhydrous Acetonitrile
Coupling: A 6 minute coupling time is recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

Base excision repair (BER) is one of the most studied repair mechanisms. In this pathway, DNA glycosylases recognize the damaged bases and catalyze their excision through hydrolysis of the N-glycosidic bond. Attempts to understand the structural basis for DNA damage recognition by DNA glycosylases have been hampered by the short-lived association of these enzymes with their DNA substrates. To overcome this problem, the Verdine group at Harvard synthesized a pyrrolidine analog that mimics the charged transition state of the enzyme-substrate complex. When incorporated into double-stranded DNA, they found the pyrrolidine analog (PYR), introduced as the Pyrrolidine-CE Phosphoramidite, forms an extremely stable complex with the DNA glycosylase AlkA, exhibiting a dissociation constant in the pM range and potently inhibited the reaction catalyzed by the enzyme

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Pyrrolidine-CE Phosphoramidite

Pyrrolidine-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 19.2: Pyrrolidine CE Phosphoramidite: a new base excision repair inhibitor

 


If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pyrrolidine-CE Phosphoramidite

Pyrrolidine-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1915-90 100µmoles .084grams 1 20 12 7.5 5.45 4 1
10-1915-95 50µmoles .042grams .5 3.33 2 1.25 .91 .67 .17
10-1915-02 0.25grams .25grams 2.97 85.67 51.4 32.13 23.36 17.13 4.28
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1915-90 100µmoles .084grams 1.5 .07 23.6 14.75 10.73 1.48
10-1915-95 50µmoles .042grams .75 .07 8.6 5.38 3.91 .54
10-1915-02 0.25grams .25grams 4.43 .07 82.2 51.38 37.36 5.14
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1915-90 100µmoles .084grams 1.5 .07 25.2 15.75 11.45

10-1915-95 50µmoles .042grams .75 .07 10.2 6.38 4.64

10-1915-02 0.25grams .25grams 4.43 .07 83.8 52.38 38.09

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200