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Oligo Synthesis

Oligo Synthesis : CEPs

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Psoralen C2 Phosphoramidite

Psoralen C2 Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1982-02Psoralen C2 Phosphoramidite 0.25g £396.00£364.32Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1982-90Psoralen C2 Phosphoramidite 100µmoles £156.00£143.52Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Psoralen C2 Phosphoramidite

Psoralen C2 Phosphoramidite

Glen Research

Psoralen C2 Phosphoramidite

Structure

Catalog Number: 10-1982-xx

Description: Psoralen C2 Phosphoramidite

2-[4'-(hydroxymethyl)-4,5',8-trimethylpsoralen]-ethyl-1-O-(2-cyanoethyl)-
(N,N-diisopropyl)-phosphoramidite
Formula: C26H35N2O6P M.W.: 502.55 F.W.: 364.29

Diluent: Anhydrous Acetonitrile
Coupling: 10 minute coupling time recommended.
Deprotection: Mild: 0.4M Methanolic NaOH 17hr @ RT or ammonium hydroxide 24h @ RT.Note: NaOH is not compatible with dmf protecting groups.Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

PSORALEN LABELLING

Psoralen C2 at the 5’-terminus of an oligonucleotide serves effectively as a cross-linking reagent in double-stranded oligonucleotides. The 6 atom spacer arm of Psoralen C6 allows cross-linking with a triplex oligonucleotide strand.

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Psoralen C2 Phosphoramidite

Psoralen C2 Phosphoramidite

Glen Research

FREQUENTLY ASKED TECHNICAL QUESTION

QUESTION: Which should I use - psoralen C2 or psoralen C6?

RESPONSE:Psoralen C2 is designed to crosslink to a T residue adjacent to the 3'-terminus of the opposite strand of double stranded DNA. Psoralen C6 is intended to fulfill the same purpose but, with the longer spacer, crosslinks to the triple strand of triplex DNA.

Max excitation=330nm, observed at max 395nm

QUESTION: What is the sequence requirement for psoralen mediated cross-linking in double stranded DNA?

RESPONSE:Psoralens are a class of naturally occurring heterocyclic compounds which intercalates between bases in double-stranded or triple stranded DNA. Upon exposure to long wavelength light (350 nm) Psoralen forms covalent linkages to Thymidine (cyclobutane linkage). Psoralen is a bifunctional reagent and can form either a monoadduct, linking one adjacent thymidine on the same or complimentary strand, or a diadduct, linking adjacent thymidines on the same or complimentary strands. Diadducts formed between adjacent thymidines are photoreversable with short wavelength UV light (254 nm).

Type of Adduct

Optimal Sequence

Cross-linking

Reversing



Wavelength

Wavelength

Monoadduct

5’-Psoralen-XAX-XXX-

350 nm

NA

Diadduct

5’-Psoralen-TAX-XXX-

350 nm

254 nm

Note: For adducts between double-stranded DNA use Psoralen C210-1982.
For adducts between triple-stranded DNA use Psoralen C6 which has a longer linker arm 10-1983.

REFERENCE(S):
1. U. Pieles and U. Englisch, Nucleic Acids Res., 1989, 17, 285.

 

QUESTION: What are the relative extinction coefficients of 5'-Fluorescein, Hex and Tet etc.. at 260 nm and their Lambda max?

RESPONSE:Please see http://www.glenresearch.com/Technical/Extinctions.html

REFERENCE(S):
Oligonucleotide Properties Calculator; http://www.basic.northwestern.edu/biotools/oligocalc.html

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Psoralen C2 Phosphoramidite

Psoralen C2 Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1982-02 0.25grams .25grams 4.97 152.33 91.4 57.13 41.55 30.47 7.62
10-1982-90 100µmoles .05grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1982-02 0.25grams .25grams 7.42 .07 142 88.75 64.55 8.88
10-1982-90 100µmoles .05grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1982-02 0.25grams .25grams 7.42 .07 143.6 89.75 65.27

10-1982-90 100µmoles .05grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200