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Oligo Synthesis

Oligo Synthesis : CEPs

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pdU-CE Phosphoramidite

pdU-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1054-02pdU-CE Phosphoramidite 0.25g £191.00 Quantity Add to Order
10-1054-05pdU-CE Phosphoramidite 0.5g £373.00 Quantity Add to Order
10-1054-90pdU-CE Phosphoramidite 100µmoles £64.00 Quantity Add to Order

Description

pdU-CE Phosphoramidite

Structure

Catalog Number: 10-1054-xx

Description: pdU-CE Phosphoramidite

5'-Dimethoxytrityl-5-(1-Propynyl)-2'-deoxyUridine,3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C42H49N4O8P M.W.: 768.85 F.W.: 328.22

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: Similar to dA,C,G,T-CE Phosphoramidites
Please Note: This product is covered by patents or patents pending owned by Isis Pharmaceuticals, Inc. ("Isis"). Purchase of this product includes a limited license to use this product solely for internal research. This license specifically excludes (and you have no right to use this product for): (a) therapeutic or diagnostic applications (including products or services that incorporate this product), (b) any in vivo toxicity/safety study in support of an investigational new drug application (or foreign counterpart), (c) resale (including sale of any products or services that incorporate this product) or (d) gene functionalization activities (including products or services that incorporate data derived from gene functionalization activities) if such activities have commercial application, any and all of which require a separate license from Isis. Neither this product nor any product created through its use may be used in human clinical trials.
Please see Terms and Conditions of Use

C-5 PROPYNE DERIVATIVES AND G-CLAMP

Substitution of C-5 propynyl-dC (pdC) for dC and C-5 propynyl-dU (pdU) for dT are effective strategies to enhance base pairing. Using these base substitutions, duplex stability and melting temperatures are raised by the following amounts: C-5 propynyl-C 2.8° per substitution; C-5 propynyl-U 1.7° per substitution. AP-dC (G-clamp) substitutes for dC and is another very important modified nucleoside that enhances hybridization by 7.5° per substitution. The ability of these modified bases to enhance binding while maintaining specificity has proven useful in antisense research and in the synthesis of high affinity probes. AP-dC is also a fluorescent nucleoside and should find uses in DNA structural research.

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Notes

QUESTION: What are the extinction coefficients for propynyl-dC and dU? Also Halogenated dC and dU derivatives? Others?

RESPONSE:See: Table of Extinction Coefficients

REFERENCE(S):
B. Froehler, Gilead
M. Powell
R. Somers


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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1054-02 0.25grams .25grams 3.25 95 57 35.63 25.91 19 4.75
10-1054-05 0.5grams .5grams 6.5 203.33 122 76.25 55.45 40.67 10.17
10-1054-90 100µmoles .077grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1054-02 0.25grams .25grams 4.85 .07 90.6 56.63 41.18 5.66
10-1054-05 0.5grams .5grams 9.71 .07 187.8 117.38 85.36 11.74
10-1054-90 100µmoles .077grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1054-02 0.25grams .25grams 4.85 .07 92.2 57.63 41.91

10-1054-05 0.5grams .5grams 9.71 .07 189.4 118.38 86.09

10-1054-90 100µmoles .077grams 1.5 .07 25.2 15.75 11.45

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Related products

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