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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Pac-dA-Me Phosphoramidite

Pac-dA-Me Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1301-02Pac-dA-Me Phosphoramidite 0.25g £30.00£27.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1301-05Pac-dA-Me Phosphoramidite 0.5g £40.00£36.80Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1301-10Pac-dA-Me Phosphoramidite 1.0g £80.00£73.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Pac-dA-Me Phosphoramidite

Pac-dA-Me Phosphoramidite

Glen Research

Pac-dA-Me Phosphoramidite

Structure

Catalog Number: 10-1301-xx

Description: Pac-dA-Me Phosphoramidite

5'-Dimethoxytrityl-N-phenoxyacetyl-2'-deoxyAdenosine,
3'-[methyl-(N,N-diisopropyl)]-phosphoramidite
Formula: C46H53N6O8P M.W.: 848.93 F.W.: 327.23 (Methyl triester) FW: 313.21 (Phosphodiester)

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer. To avoid any exchange of the iPr-Pac group on the dG with acetyl, use UltraMild Cap Mix A (40-4210-xx/40-4212-xx).
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature to leave the methyl phosphotriester intact. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

METHYL PHOSPHORAMIDITES

For many years, Glen Research has supplied methyl phosphoramidites in addition to ?-cyanoethyl (CE) phosphoramidites for the few situations where the more labile cyanoethyl group is not an advantage. Some of our customers, probably remembering that the methyl group was removed specifically with thiophenol, have tried to use these monomers to prepare the interesting, uncharged, and nuclease-resistant methyl phosphotriester linkage. Unfortunately, this linkage is labile to ammonium hydroxide and the regular phosphodiester linkage is formed (along with a small amount of chain scission). Now we offer UltraMild methyl phosphoramidites for this application. Oligos produced from these monomers can be deprotected with potassium carbonate in methanol to produce methyl phosphotriester linkages. Since these linkages are diastereomeric and uncharged, the oligos may be hard to handle. Consequently, it is likely that chimeras will be produced using these monomers along with the regular UltraMild CE phosphoramidites. If many dG residues are included in the oligonucleotide, we recommend the use of phenoxyacetic anhydride (PAc2O) in Cap A. This modification removes the possibility of exchange of the isopropyl-phenoxyacetate (iPr-Pac) protecting group on the dG with acetate from the acetic anhydride capping mix.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pac-dA-Me Phosphoramidite

Pac-dA-Me Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 14.1: PRODUCT UPDATE - HOW ARE METHYL TRIESTER LINKAGES PREPARED?

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pac-dA-Me Phosphoramidite

Pac-dA-Me Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1301-02 0.25grams .25grams 2.94 84.67 50.8 31.75 23.09 16.93 4.23
10-1301-05 0.5grams .5grams 5.89 183 109.8 68.63 49.91 36.6 9.15
10-1301-10 1.0gram 1grams 11.78 379.33 227.6 142.25 103.45 75.87 18.97
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1301-02 0.25grams .25grams 4.4 .07 81.6 51 37.09 5.1
10-1301-05 0.5grams .5grams 8.79 .07 169.4 105.88 77 10.59
10-1301-10 1.0gram 1grams 17.58 .07 345.2 215.75 156.91 21.58
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1301-02 0.25grams .25grams 4.4 .07 83.2 52 37.82

10-1301-05 0.5grams .5grams 8.79 .07 171 106.88 77.73

10-1301-10 1.0gram 1grams 17.58 .07 346.8 216.75 157.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200