sbeadex™ pathogen nucleic acid purificaton kit
High-throughput, high-quality DNA and RNA purification for detection of multiple pathogens
Pathogen testing protocols (e.g. for COVID-19, influenza or easy-to-lyse bacterial pathogens) require fast, reliable, high-yield, high-quality and easily automatable sample preparation methods.
sbeadex™ Pathogen Nucleic Acid Purification Kits with their optimised chemistry meet these requirements and deliver excellent yields of highly purified nucleic acids from various sample material that can be used in many downstream applications such as PCR/qPCR, RT-PCR/qRT-PCR, and NGS. The purification procedure can be fully automated and is compatible with many robotic platforms.
The sbeadex™ Pathogen Nucleic Acid Purification Kit is a simple renaming of our proven sbeadex Viral RNA Purification Kit to reflect its continuously expanding usability to purify good yields of high-quality DNA and RNA from a variety of viral and bacterial pathogens.
Please see Table 1 for a list of viral and bacterial pathogens and compatible matrices that have been tested so far.
For Research Use Only. Not for use in diagnostic procedures.
Key benefits
* Qualified protocol for viral and bacterial nucleic acid purification
* Compatible with most popular robotic platforms, including the Kingfisher™ and the high-throughput oKtopure™ instrument
* Fast – both short (25 min) and standard (45 min) protocols available
* High-throughput batch sizes (up to 10,000 purifications)
* No organic extraction, alcohol precipitation or centrifugation steps
* Water-based wash buffers reduce solvent carryover, resulting in absence of PCR inhibitors in final eluate
* High nucleic acid quality for all downstream applications including PCR/qPCR and NGS
* Ready-to-use kits with high stability (customised and bulk size kits are also available)
Please see the table below for a list of viral and bacterial pathogens and compatible matrices that have been tested so far.
| Pathogen | Matrix |
| SARS-CoV-2 | Swab and Sputum |
| Influenza A H3N2 | Swab* |
| Influenza B | Swab* |
| Novel Influenza A H1N1 | Swab* |
| Respiratory Syncytial Virus (subtype A) | Swab* |
| Respiratory Syncytial Virus (subtype B) | Swab* |
| Adenovirus 4 | Swab* |
| Bordetella pertussis | Swab** |
| Chlamydophila pneumoniae | Swab** |
| Chlamydophila psittaci | Swab** |
| Coxiella burnetii | Swab** |
| Legionella pneumophila | Swab** |
| Mycoplasma pneumoniae | Swab** |
| Mycobacterium tuberculosis | Sputum*** |
Table 1: Pathogens that have already been tested using the sbeadex Pathogen Nucleic Acid Purification Kit.
Scientific data have been generated using AccuPlex™ Reference Material from LGC SeraCare in the background of human swab and sputum matrix. Furthermore, *Amplirun Total Respiratory Viral Panel Control (Swab, MBTC020), **Amplirun Total Atypical bacterial Pneumonia Control (Swab, MBTC022-R) and ***Amplirun Total MTB Control (Sputum, MBTC013) from Vircell S.L. were used. Subsequent detection was performed using different (RT-)qPCR systems. For details, please contact our technical support.
Note: Other sample matrices may also be used with this kit. Further testing on other pathogens and matrices is in progress and this table will be updated accordingly.
Product info
sbeadex Pathogen Nucleic Acid Purification Kits are magnetic-bead based kits that are validated for research use only. The sbeadex pathogen nucleic acid purification portfolio offers products for all scales of nucleic acid preparation from buccal swab and sputum samples, eluting into 100 µL buffer.
DNA/RNA preparation protocol
sbeadex beads allow efficient and high-quality purification of small and large DNA and RNA fragments within 45 minutes without centrifugation steps. The beads are double coated, resulting in a unique dual binding protocol that allows elution of ultrapure nucleic acids into water, reducing solvent carryover:
|
|
sbeadex Pathogen Nucleic Acid
Purification Kit
|
Competitor A
|
|
|
N
|
Average Cq
|
StDev
|
Average Cq
|
StDev
|
|
Clean up sample
|
12
|
30.35
|
0.14
|
33.80
|
0.15
|
|
Direct spike-in sample
|
3
|
31.00
|
0.44
|
33.80
|
0.12
|
Figure 1: Demonstration of no loss of RNA during the sbeadex Pathogen Nucleic Acid Purification Kit protocol. 500 copies of a 2019-COVID artificial RNA reference material were added to a sample (nasopharyngeal swab) and the sample processed with the sbeadex Pathogen Nucleic Acid Purification Kit. SARS-CoV-2 was detected in this preparation and a directly spiked positive control sample via a RT-qPCR at two separate Biosearch Technologies sites (reaction components described below). The concordant Cq values from both samples demonstrate no loss of sample due to the extraction step.
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