Find the most stable reference genes with TATAA Biocenter's Reference Gene Panel - Short assay
Use the Reference Gene Panel - Short assay for Human to determine
the optimal reference genes for your samples, without spending
unnecessary time on primer design. The short assay includes 9 reference
genes with shorter amplicons designed to work even on partly degraded
material. The panel is available for probe and SYBR® based systems.
Description
For all gene expression studies using qPCR, it is necessary to
compensate for differences between samples due to material losses,
differences in RT yields and PCR inhibition. Normalization should
include a reference gene, but can also be complemented by identical
sample-input amounts. The reference gene should have constant expression
in all the samples compared. There is no universal control gene,
expressed at a constant level under all conditions and in all tissues,
hence the choice of reference gene should be validated for each new
study.
The best way to choose the proper reference gene(s) is by running a
panel of potential genes on a number of representative test samples. The
most appropriate gene(s) for normalization are chosen in each case.
When you have selected the appropriate reference gene(s) for your
study, you can purchase them individually as single assays here in
TATAA's web shop.