Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Total RNA

Specialised kits for Total RNA purification for various sources.

QuickExtract™ RNA Extraction Kit

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR, both end-point and real-time.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
QER090150 QuickExtract™ RNA Extraction Kit50ml (500 Extraction) £428.00 Quantity Add to Order

QuickExtract™ RNA Extraction Kit

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR, both end-point and real-time.

BioSearch Tech (Lucigen/Epicentre)

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR (both end-point and real-time). The single-tube system requires only vortex mixing to lyse the cells, and prepare the RNA for cDNA synthesis. The result is easy processing of one to hundreds of samples in minutes, with no sample loss or toxic organic solvents. The QuickExtract RNA Extraction Solution works with cultured adherent and suspension cells including buccal cells, and has been tested on human, mouse, rat, E. coli, and S. aureus cell cultures. It is not suitable for tissue samples or plant samples. An optional DNase I treatment may improve certain downstream applications.

QuickextractRNAfig1 Figure 1. End-point RT-PCR of different regions of a 14 kb message using HeLa cell extract with the QuickExtract™ RNA Extraction Kit. A sample containing 105 HeLa cells was lysed in 100 µl of QuickExtract RNA Extraction Solution by vortex mixing. The lysate was reverse transcribed with the MMLV Reverse Transcriptase 1st Strand cDNA Synthesis Kit using standard conditions and random primers. The cDNA was then amplified with six primer sets to p532 using the FailSafe™ PCR System. Lane M, 100 bp ladder; Lane 1, 12984 to 13892; lane 2, 9406 to 10202; lane 3, 5194 to 5802; lane 4, 4191 to 4690; lane 5, 2280 to 2676; and lane 6, 1029 to 1329
QuickextractRNAfig2 Figure 2. Comparative yield of RT-PCR product with different RNA extraction kits. Lysates were prepared according to manufacturers' instructions and used as template to produce cDNA using the MMLV RT 1st Strand cDNA Synthesis Kit, followed by PCR using the FailSafe™ PCR System with primers for the ALDOA gene. Products were separated on a 2% agarose gel and stained with SYBR® gold. Lane M, 100 bp ladder; lanes 1 and 2, QuickExtract™ RNA Extraction Kit; lanes 3 and 4, kit from Vendor 1; lanes 5 and 6, kit from Vendor 2.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ RNA Extraction Kit

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR, both end-point and real-time.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: QuickExtract™ RNA Extraction Kit

QuickExtract™ RNA Extraction Kit Protocol

(catalogue number QER09015 / QER090150 )

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ RNA Extraction Kit

The QuickExtract™ RNA Extraction Kit is a fast, simple way of preparing RNA for RT-PCR, both end-point and real-time.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Preparation of RNA from cultured adherent and suspension cells for RT-PCR.

Benefits

  • Single-tube system.
  • Prepare lysate in minutes.
  • No toxic organic solvents.
  • Suitable for high-throughput applications.
  • Optional DNase I treatment.

 

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200