Competent Cells
Range of Chemically and Electrocompetrent cells for your molecular cloning needs
Overview of the range
CopyCutter™ EPI400™ Electrocompetent and Chemically Competent E. coli
Highly stable and competent cells for unstable DNA and keeping low copy numbers
CopyCutter™ EPI400™ E.coli* cells were developed to significantly lower the copy number of a wide variety of common vectors so that you can more readily clone unstable DNA sequences. DNA that is unstable at high-copy number often codes for a protein that inhibits cell growth or contains AT- and GC-rich sequences or sequences with strong secondary structure. The CopyCutter™ EPI400™ cell line was derived from our high-transformation efficiency phage T1-resistant TransforMax™ EC100™ E. coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e.g., pUC- and pET-type vectors).
TransforMax™ EC100™ Electrocompetent and Chemically Competent E. coli
Highly competent cells for general cloning applications
Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
Achieve high efficiencies with convenient chemically competent format: >5 × 108 cfu/µg pUC19 DNA.
Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations.
TransforMax™ EPI300™ Electrocompetent and Chemically Competent E. coli
Compentent cells for standard cloning for large inserts and Gibson assembly electroporation
Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.
Generate clones with inducible copy number using CopyControl™ vectors.
Achieve high transformation efficiencies: >1 × 1010 cfu/µg pUC19 DNA.
Increase Gibson Assembly® colony counts with high-efficiency electroporation.
Endura Competent Cells
Clone and maintain unstable or repetitive sequences and lentiviral libraries with high efficiency. Ideal for
GeCKO CRISPR library transformation. The kit includes Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube) or DUOs (2 transformations per tube). Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately.
E. cloni 10G Elite Electrocompetent Cells
E. cloni 10G Elite Electrocompetent Cells, (SOLOs) Clone, construct libraries, transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications.