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TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
TY0261HTypeOne™ Restriction Inhibitor100µg £172.00£172.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

DNA transformation can be difficult to achieve in many bacterial strains due to the presence of one or more restriction and modification (R-M) systems that cleave unmodified DNA that is recognized as "foreign." TypeOne™ Restriction Inhibitor* provides a powerful method for increasing transformation efficiencies in bacterial strains with type I R-M systems. Developed as a unique preparation of a phage protein called ocr,1 TypeOne Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage. 

Table 1. Effect of Typeone™ Restriction Inhibitor on transforation efficencies

Strain(Type I R-M System)Typeone™ InhibitorType of DNA or Transposome™Recombinants per µg DNA
S. typhimurium LT2 (StyL TIII)   pUC19 (100pg) 3.0 x 106
S. typhimurium LT2 (StyL TIII) X pUC19 (100pg) 3.0 x 108
E. coli MG1655 (EcoK1)   48kb fosmid (50ng) 3.0 x 103
E. coli MG1655 (EcoK1) X 48kb fosmid (50ng) 1.4 x 106
S. typhimurium LT2 (StyL TIII)   EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ (1µl) 1.3 x 104
S. typhimurium LT2 (StyL TIII) X EZ-Tn5™ <R6Kγori/KAN-2>Tnp Transposome™ (1µl) 1.0 x 106

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TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

Protocols for: TypeOne™ Restriction Inhibitor

TypeOne™ Restriction Inhibitor Protocol

(catalogue number TY0261H)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

References

  1. Walkinshaw, M.D. et al. (2002) Molec. Cell 9, 187.
  2. Hoff man, L. et al. (2002) EPICENTRE Forum 9(2), 8.
  3. Murray, N.E. et al. (2000) Microbial. Molec. Biol. Rev. 64, 412.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TypeOne™ Restriction Enzyme Inhibitor

TypeOne ™ Restriction Enzyme Inhibitor is readily electroporated into cells along with transforming DNA. In vivo, the protein acts as a molecular decoy that blocks the DNA-binding site of type I R-M enzymes and inhibits DNA cleavage

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Increase the transformation efficiency of unmodified DNA2 by blocking type I R-M systems, which are widespread in Eubacteria and Archaebacteria.3

Benefits

  • Easy to use: Simply add TypeOne Restriction Inhibitor to unmodified DNA or a Transposome and electroporate using standard methods.
  • Blocks all known families of type I R-M enzymes1 and can therefore be used to increase transformation efficiencies in a variety of bacterial cells.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200