Cambio - Excellence in Molecular Biology

Transposomics

Transposomics: Transposomics ™

EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit completely sequences cDNA or genomic clones in plasmid, cosmid, fosmid, or BAC vectors without subcloning or primer walking

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
EC10010TransforMax™ EC100™ Electrocompetent E. coli*10X100µl £253.00£253.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
EZI03T7EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit10 reactions £471.00£471.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit completely sequences cDNA or genomic clones in plasmid, cosmid, fosmid, or BAC vectors without subcloning or primer walking

BioSearch Tech (Lucigen/Epicentre)

 

Random insertion of T7 promoter to drive gene expression with T7 RNA polymerase

  • Insert T7 promoter randomly into any DNA sequence in vitro
  • Isolate positive clones with the selectable kanamycin marker
  • Express resulting products in vivo or in vitro
  • Minimize insertion bias with the hyperactive Tn5 system, known for highest level of randomness

Applications

  • Random insertion of a single T7 RNA Polymerase promoter to synthesize RNA from any region of cloned DNA.

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit* provides an easy and reliable method to randomly insert a transposon containing a phage T7 RNA polymerase promoter into any DNA molecule in vitro. The transposon end has no termination sequences, so RNA can be produced from chosen insertion clones by in vitro transcription from the T7 RNA polymerase promoter using, for example, any of Epicentre's T7 RNA Polymerase Transcription Kits. RNA can also be generated for in vivo expression studies in cells having an inducible T7 RNA polymerase gene

 

 

Figure 1 (click to enlarge). RNA transcripts can be produced from insertion clones in vitro or in vivo using the EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit.

 

*Covered by issued and/or pending patents, exclusively licensed or assigned to Epicentre® (an Illumina® Company).



If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit completely sequences cDNA or genomic clones in plasmid, cosmid, fosmid, or BAC vectors without subcloning or primer walking

BioSearch Tech (Lucigen/Epicentre)

Protocols for: EZ-Tn5™ <T7/KAN-2> Promoters Insertion Kit 

EZ-Tn5™ <T7/KAN-2> Promoters Insertion Kit Protocol

(catalogue number EZI03T7) 

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit completely sequences cDNA or genomic clones in plasmid, cosmid, fosmid, or BAC vectors without subcloning or primer walking

BioSearch Tech (Lucigen/Epicentre)

*TransforMax™ EC100™ Electrocompetent Cells are recommended for high-efficiency, non-size-biased cloning of DNA containing EZ-Tn5™ Transposon insertions.

For Research Use Only. EZ-Tn5™ products covered by issued and pending patents

   

Epicentre® Forum Get your Epicentre® Forum - hot off the press!
Click here for direct link to Forum listings

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit

The EZ-Tn5™ <T7/KAN-2> Promoter Insertion Kit completely sequences cDNA or genomic clones in plasmid, cosmid, fosmid, or BAC vectors without subcloning or primer walking

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Random insertion of a single T7 RNA Polymerase promoter to synthesize RNA from any region of cloned DNA.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200