The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).
||1.4 x 108
||1.4 x 1010
||1.3 x 107
||4.3 x 106
||1.3 x 109
||9.2 x 105
||3.0 x 108
|145-kb BAC Clone
||7 x 107
|13.1-kb clone directly from a ligation reaction
||2.2 x 105
||2.1 x 107
Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG
TransforMax EC100 Electrocompetent E. coli
- Transformation efficiency of >1 x 1010 cfu/µg of pUC19.
TransforMax EC100 Chemically Competent E. coli
- Transformation efficiency of >5 x 108 cfu/µg of pUC19.
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