Cambio - Excellence in Molecular Biology

Molecular Cloning Kits

Molecular Cloning Kits: Competent Cells

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
CC02810TransforMax™ EC100™ Chemically competent E. coli10 X 50ul £128.00£128.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
EC10010TransforMax™ EC100™ Electrocompetent E. coli10 X 100µl £253.00£253.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

The highly versatile TransforMax™ EC100™ E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).

DNA

TransforMax™ EC100™ Chemically Competent E. coli

TransforMax™ EC100™ Electrocompetent E. coli

pUC19 1.4 x 108 1.4 x 1010
8.1-kb Clone 1.3 x 107 Not tested
13.1-kb Clone 4.3 x 106 1.3 x 109
23.1-kb Clone 9.2 x 105 3.0 x 108
145-kb BAC Clone Not tested 7 x 107
13.1-kb clone directly from a ligation reaction 2.2 x 105 2.1 x 107

Table 1. Comparison of the transformation efficiencies of TransforMax™ EC100™ E. coli with a variety of DNAs. Transformations were performed using 50 µl of competent cells and either supercoiled DNAs of the indicated sizes or a 1-µl aliquot from a standard 10-µl ligation reaction. Results shown are in cfu/µg of DNA and are the average transformation efficiencies obtained from several trials.

Genotype

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (StrR) nupG

TransforMax EC100 Electrocompetent E. coli

  • Transformation efficiency of >1 x 1010 cfu/µg of pUC19.

TransforMax EC100 Chemically Competent E. coli

  • Transformation efficiency of >5 x 108 cfu/µg of pUC19.




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

Protocols for: TransforMax™ EC100™ Electrocompetent E. coli & TransforMax™ EC100™ Chemically competent E. coli

TransforMax™ EC100™ Electrocompetent E. coli Protocol

(catalogue number EC10010)

TransforMax™ EC100™ Chemically competent E. coli Protocol

(catalogue number CC02810)

Please note: all protocols off site are the responsibility of the product supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

Citations for TransforMax™ EC100™ Electrocompetent E. coli and
TransforMax™ EC100™ Chemically Competent E. coli

  1. Uhlich, G. A. (2009) KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157 : H7, Microbiology 155 , 3589-3598.
  2. Uhlich, G. A., et al. (2009) The CsgA and Lpp Proteins of an Escherichia coli O157:H7 Strain Affect HEp-2 Cell Invasion, Motility, and Biofilm Formation, Infect. Immun. 77 , 1543-1552.
  3. Vaezeslami, S., et al. (2007) Site-Directed Mutagenesis Studies of Tn5 Transposase Residues Involved in Synaptic Complex Formation, J. Bacteriol. 189 , 7436-7441.
  4. Kloosterman, W. P., et al. (2006) Cloning and expression of new microRNAs from zebrafish, Nucleic Acids Res. 34 , 2558-2569.
  5. Sanseverino, J., et al. (2005) Use of Saccharomyces cerevisiae BLYES Expressing Bacterial Bioluminescence for Rapid, Sensitive Detection of Estrogenic Compounds, Appl. Envir. Microbiol. 71 , 4455-4460.
  6. Dorsey, C. W., et al. (2004) The siderophore-mediated iron acquisition systems of Acinetobacter baumannii ATCC 19606 and Vibrio anguillarum 775 are structurally and functionally related, Microbiology 150 , 3657-3667.
  7. Wyborn, N. R., et al. (2004) Properties of haemolysin E (HlyE) from a pathogenic Escherichia coli avian isolate and studies of HlyE export, Microbiology 150 , 1495-1505.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Great for general cloning applications where high transformation efficiencies are desired
  • Routine cloning of DNA up to 200 kb.

Benefits

  • High transformation efficiency with clones of all sizes, including BAC clones (Table 1).
  • lacZΔM15 for blue/white screening of recombinants.
  • Restriction minus [mcrA, Δ(mrr-hsdRMS-mcrBC)] enables efficient cloning of methylated DNA.
  • Endonuclease minus (endA1) to ensure high yields of DNA.
  • Recombination minus (recA1) for greater stability of large cloned inserts

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TransforMax™ EC100™ Electro- and chemically competent E. coli

TransforMax EC100 electrocompetent E. coli have the highest transformation efficiency, and are ideal for cloning applications

BioSearch Tech (Lucigen/Epicentre)

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200