Cambio - Excellence in Molecular Biology

Enzymes for Molecular Biology

Enzymes for Molecular Biology: Heat labile & Salt Tolerant Enzymes

New range of enzymes for 2019. Our new range of enzymes include novel enzymes specifically designed or engineered to be amazingly tolerant to high salt concentrations, function at very low temperatures and heat inactivated.

Cryophile Shrimp Alkaline Phosphatase PCR cleanup kit

• Removes excess primers and dNTPs • Fast 15 minute protocol • Add directly to PCR product • 100% Sample Recovery • Scalable for different reaction sizes • Easy to automate

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-1739-100Cryophile Shrimp Alkaline Phosphatase PCR Cleanup100 rxn £65.00 Quantity Add to Order
CA-1739-2000Cryophile Shrimp Alkaline Phosphatase PCR Cleanup2000 rxn £750.00 Quantity Add to Order
CA-1739-500Cryophile Shrimp Alkaline Phosphatase PCR Cleanup500 rxn £220.00 Quantity Add to Order

Cryophile Shrimp Alkaline Phosphatase PCR cleanup kit

• Removes excess primers and dNTPs • Fast 15 minute protocol • Add directly to PCR product • 100% Sample Recovery • Scalable for different reaction sizes • Easy to automate

Cambio

Cryophile Shrimp Alkaline Phosphatase PCR Cleanup 

A quick, easy and reliable enzymatic reagent for PCR cleanup

 

  • Removes excess primers and dNTPs
  • Fast 15 minute protocol
  • Add directly to PCR product
  • 100% Sample Recovery
  • Scalable for different reaction sizes
  • Easy to automate
  • Enzymatic cleanup of PCR products


The Cryophile Shrimp Alkaline Phosphatase PCR Cleanup method utilizes two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I). The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling sequencing and other downstream applications. The reagents are active in commonly used PCR buffers and eliminates the need for buffer exchange.

 

Protocol
The Cryophile Shrimp Alkaline Phosphatase PCR Cleanup components can be added directly into the PCR product, followed by 5 minute incubation at 37°C. Inactivation of both enzymes is achieved in only 10 minutes at 80°C. PCR  products are immediately ready for downstream applications without any need for further processing.

 

 

 

 

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