Cryophile DNase HL65 (heat inactivatable) is a native form of double-stranded DNase that is rapidly and completely inactivated at 65°C for 15 minutes. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation. See also Cryophile dsDNase.
The enzyme requires 1 mM DTT and pH ≥= 8 for complete inactivation. This makes theproduct very useful for removal of DNA from RNA preparations, since the enzyme may be heat inactivated at temperatures causing only minimal auto-degradation of RNA temperatures. In comparison Cryophile DNase is inactivated at 58°C with a pH above 8.0.)
Heat Inactivation Cryophile dsDNAse HL65
The dsDNase can be heat inactivated by heat treatment at 15 min at 65°C, or 20 min at 60°C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
Specifications
• Unit definition
One Unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCl2.
• Specific activity
Ca. 400 000 Kunitz Units/mg.
• Activity
The dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.
• Storage buffer
20 mM Tris-HCl pH 7.5, 2 mM MgCl2, 10 mM NaCl, 0.01% (v/v) Triton X-100, 50% (v/v) glycerol.
• Purity
dsDNase is purified to apparent homogeneity.
• Storage
Minimum shelf life is 3 years at -20°C. Storage at 4°C is possible for at least 6 months. The enzyme also tolerates multiple freeze-thaw cycles.
Heat Inactivation dsDNAse HL65
The dsDNase can be heat inactivated by heat treatment at 15 min at 65°C, or 20 min at 60°C. The enzyme requires 1 mM DTT and pH ≥ 8 for complete inactivation.
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