MonsterScript™ Reverse Transcriptase is a tmagnesium-dependen thermostable reverse transcriptase that completely lacks RNase H activity. The enzyme's lack of RNase H activity contributes to its ability to make longer cDNAs and more complete full-length libraries of first-strand cDNA molecules compared to other reverse transcriptases. EPICENTRE scientists have demonstrated reverse transcription of 15-kb RNA templates using MonsterScript™ Reverse Transcriptase (Fig. 1).
The MonsterScript 1st-Strand cDNA Synthesis Kit includes MonsterScript RT PreMix, which contains optimized concentrations of dNTPs, Mg2 , and betaine.* Betaine reduces pausing and stops by the reverse transcriptase, enabling improved reverse transcription through difficult sequences, such as regions of high GC content. The kit also contains both an oligo(dT)-containing primer and random nonamer primers.
MonsterScript™ Reverse Transcriptase is free of detectable contaminating DNA exo- and endonuclease and RNase activities.
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