5'-Biotin Phosphoramidite
[1-N-(4,4'-Dimethoxytrityl)-biotinyl-6-aminohexyl]-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite
Description
Biotin-dT can replace dT residues
within the oligonucleotide sequence. 5'-Biotin phosphoramidite can be
added ONLY ONCE to the 5'-terminus of an oligonucleotide. However, the
DMT group on the biotin can be used in RP cartridge and HPLC
purification techniques. PC Biotin is a photocleavable 5'-biotin
phosphoramidite. BiotinTEG CPG and Protected BiotinLC Serinol CPG are
designed for the direct synthesis of oligonucleotides containing biotin
at the 3' terminus.
Desthiobiotin is a biotin analogue that exhibits lower binding to
biotin-binding proteins such as streptavidin. This biotin analogue is
lacking the sulfur group from the molecule and has a dissociation
constant (Kd) several orders of magnitude less than biotin/streptavidin.
As a result, biomolecules containing desthiobiotin are dissociated
from streptavidin simply in the presence of buffered solutions of
biotin. We offer desthiobiotinTEG phosphoramidite and the corresponding
CPG.
Details
Usage
- Coupling: 2 minute coupling time recommended
- Deprotection:
5'-Biotin is slow to detritylate. If the final DMT-group is to be
removed on the synthesizer, we recommend a second deblocking step. If
the final DMT-group is left on to aid in cartridge purification, we
recommend that the oligo is left in contact with the TFA solution for 10
minutes.
| Specifications |
|---|
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability | 1-2 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-5950-02 |
0.25 g |
.25grams |
2.95 |
85 |
51 |
31.88 |
23.18 |
17 |
4.25 |
| 10-5950-90 |
100 µmol |
.085grams |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-5950-95 |
50 µmol |
.042grams |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-5950-02 |
0.25 g |
.25grams |
4.41 |
0.07 |
81.8 |
51.13 |
37.18 |
5.11 |
| 10-5950-90 |
100 µmol |
.085grams |
1.5 |
0.07 |
23.6 |
14.75 |
10.73 |
1.48 |
| 10-5950-95 | 50 µmol | .042grams | 0.75 | 0.07 | 8.6 | 5.38 | 3.91 | 0.54 |
Safety Data Sheet
Glen Report 24.25: Technical Brief - Reagents for 5’-Labeling of microRNAs
Glen Report 8.21: 5'-Labelling - Fluorescein and Cyanine Dyes, Biotin
Glen Report 33-24: New Product — 5’-Biotin II Phosphoramidite
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