Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Immuno Reagents

Immuno Reagents from the Source Medicago develops and manufactures a wide range of immuno reagents, including monoclonal and polyclonal antibodies as well as antigens from bacteria, parasites and other sources plus synthetically manufactured antigens and antigen analogues. We also offer complete kits for the in vitro diagnosis of parasite diseases in rabbits and rodents. These include Carbon Immuno Assay (CIA) for Encephalitozoon cuniculi and Enzyme Linked Immunoassay (ELISA) for E. cuniculi and T. gondii. Scientists at Medicago have broad experience in the production and purification of antibodies used in the general in vitro diagnostics markets. We provide full service from design of the antigen and immunisation protocol to immunisation, harvesting, fusion, sub-cloning and production. We have developed special expertise in producing monoclonal antibodies against very small molecules (molecular weights down to 190 g/mol) by binding an antigen molecule analogue to a larger carrier protein in order to induce immune response. Our antibodies are developed in mice, rats, rabbits, goats and other animals. •Highest quality •Highly purified antigens and antibodies •Complete kits for in vitro diagnostics •Kits for CIA and ELISA techniques •Highly active antiserum .

Protein L Ligand Leakage ELISA

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-10-0027-1Protein L Ligand Leakage ELISA48 Tests £949.00 Quantity Add to Order
MC-10-0028-1Protein L Ligand Leakage ELISA XL 1 kit £1,063.00 Quantity Add to Order

Protein L Ligand Leakage ELISA

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody.

Medicago AB

FeaturesProtein L Ligand Leakage ELISA

  • Complete ready to use kit.
  • Detection and quantification of Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions.
  • Includes 12 strips (96 wells), sufficient for 48 test.

 

Product description

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody.

In this assay, the samples are boiled to separate the Ig/Ig-fragments from the Protein L in the solution, centrifuged, and incubated in the strip wells.

The bound Protein L is then detected by adding a horseradish peroxidase (HRP)-conjugated anti-Protein L IgY antibody.

Substrate is added to induce a color change which is proportional to the amount of bound Protein L from the sample. The color change is quantified spectrophotometrically at 450 nm by using a plate reader.

The amount of protein L in the sample is determined by using a standard curve prepared in a reference sample. The reference sample should have the same concentration of Ig or Ig-fragments and preferably the same buffer composition as the elution samples from the Protein L affinity chromatography medium. However, it should be prepared using another purification strategy, e.g. ion exchange chromatography. Once a reference sample is created it can be aliquoted and used for several different protein L ELISA assays.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Protein L Ligand Leakage ELISA

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody.

Medicago AB

Shipping and storage

The product is shipped cooled ( 4ºC) except the protein L reference which is shipped frozen
(-20ºC). On arrival store components cooled ( 4ºC) except the protein L reference which should be stored at -20/-80ºC:

  • Strips (96 wells), store unused strips in sealed bag at 4ºC.
  • HRP-conjugated anti Protein L pAb and EC-Blue Enhanced TMB-substrate, store protected from light.
  • Protein L reference,store in working aliquots to avoid repeated freezing/thawing.

 

Specifications

Components:

  • 12 strips (96 wells) Anti Protein L coated
  • 100x solution HRP-conjugated anti Protein L pAb
  • Protein L reference
  • EC-Blue Enhanced TMB-substrate
  • Stop Solution
  • PBS-Tween tablets

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Protein L Ligand Leakage ELISA

This ELISA-kit has been designed to detect and quantify Protein L in Immunoglobulin (Ig) or Ig-fragment containing solutions. It is a sandwich ELISA with microtiter strips coated with an affinity purified anti-Protein L IgY-antibody.

Medicago AB

Applications

  • Determination of leakage levels of protein L in protein L-medium purification  of intact immunoglobulin or immunoglobulin fragments (Ig or Ig fragments). Some fab samples may require optimization. Currently there is no protocol available for sdab molecules.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200