Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Immuno Reagents

Immuno Reagents from the Source Medicago develops and manufactures a wide range of immuno reagents, including monoclonal and polyclonal antibodies as well as antigens from bacteria, parasites and other sources plus synthetically manufactured antigens and antigen analogues. We also offer complete kits for the in vitro diagnosis of parasite diseases in rabbits and rodents. These include Carbon Immuno Assay (CIA) for Encephalitozoon cuniculi and Enzyme Linked Immunoassay (ELISA) for E. cuniculi and T. gondii. Scientists at Medicago have broad experience in the production and purification of antibodies used in the general in vitro diagnostics markets. We provide full service from design of the antigen and immunisation protocol to immunisation, harvesting, fusion, sub-cloning and production. We have developed special expertise in producing monoclonal antibodies against very small molecules (molecular weights down to 190 g/mol) by binding an antigen molecule analogue to a larger carrier protein in order to induce immune response. Our antibodies are developed in mice, rats, rabbits, goats and other animals. •Highest quality •Highly purified antigens and antibodies •Complete kits for in vitro diagnostics •Kits for CIA and ELISA techniques •Highly active antiserum .

EC-Blue® Enhanced Substrate (TMB)

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-10-9405-100EC-Blue® Enhanced Substrate (TMB)100 ml £27.00 Quantity Add to Order
MC-10-9405-1000EC-Blue® Enhanced Substrate (TMB)1000 ml £182.00 Quantity Add to Order
MC-10-9405-250EC-Blue® Enhanced Substrate (TMB)250 ml £47.00 Quantity Add to Order

EC-Blue® Enhanced Substrate (TMB)

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

Medicago AB

Product description

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

One bottle of EC Blue consists of a solution containing proprietary ready-mixed chromogen substrate reagents, pH 3.8. The solution has a straw-yellow colour at room temperature and a light greenish tint when stored at 4°C. The colour returns to normal when warmed overnight to room temperature.

 

Features 

  • Ready-to-use liquid substrate
  • Substrate for assays using horseradish peroxidase (HRP) conjugate
  • Low background
  • High sensitivity
  • Long stability

 

For laboratory use only.

 

Storage and stability

Store at 2°C to 8°C. Do not freeze and do not expose to direct sunlight. Prolonged exposure to light is not recommended. Store in an amber bottle. As some metal ions can oxidize TMB, allow only glass or plastic to come in contact with the substrate.

Never pipette directly from the bottle. Do not leave the cap off. EC-Blue Enhanced™ Reagent is stable for at least 18 months when stored at 2°C to 8°C.

 

Directions for use

No additional mixing reagents are required. The material is not dependent on room temperature.

 

Shipping and Storage

The product is stable for 12 months if stored at 2°c to 8°C.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

EC-Blue® Enhanced Substrate (TMB)

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

Medicago AB

Specifications

Appearance: Straw yellow colour at room temperature. Slightly green tint when stored at 4°C. Colour returns to yellow when stored at room temperature.

Protein content: One bottle of EC Blue consist of a solution containing chromogen substrate reagents already mixed, pH 3, 8.                     

Functional characteristics:  Functional testing shows less than 10% average standard deviation from previously approved lots of chromogen. Test performed in the same assay. Background optical densities shows less than 10% deviation from that of previously approved lots when tested in the same assay.

Storage: 2°C to 8°C

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

EC-Blue® Enhanced Substrate (TMB)

3, 3’, 5, 5’-Tetramethylbenzidine (TMB) substrate is a ready-to-use liquid substrate for HRP, specially developed for ELISA procedures. EC-Blue Enhanced™ Substrate turns deep blue (620-650 nm) in the presence of a peroxidase-labelled conjugate. Colour development can be stopped by adding an equal volume of 0.5 M H2SO4 (Article no. 10-9500-50), which turns the substrate yellow (450 nm).

Medicago AB

Applications

  • Recommended for ELISA procedures

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200