Cambio - Excellence in Molecular Biology

Lab Reagents

Lab Reagents: Smart Buffers and Reagents

Biological buffers for the life science laboratory need to meet a wide array of criteria. These include good stability, lack of toxicity, precision in pH, pKa between 6.0 and 8.0 (the region in which most biological reactions occur), minimal salt effects due to the ionic composition of the solution, free of enzymatic and hydrolytic activity and minimal participation in biological reactions. Buffers for molecular biology applications, e.g. Tris-Borate-EDTA and Tris-Acetate-EDTA, need to be free from DNAse and RNAse activity. Medicago's SmartBuffers meet all these criteria. They are manufactured using highly purified, analytical grade chemicals and are extensively quality tested. Manufacturing is done in a controlled clean environment according to GMP procedures. A high level of automation essentially avoids operator intervention and contact with the products. SmartBuffers are supplied as exactly pre-weighed tablets packed in containers or blister packs or as exactly pre-weighed powder mixes packed in sealed aluminium foil pouches. •Pre-mixed powder buffers or tablets with pre-set pH•Analytical grade reagents •Eliminates calculation, formulation and weighing errors •Dissolve-and-go for greater convenience •Stable at room temperature for at least 3 years •Guaranteed reproducibility

Tris Buffer (Tris-HCl)

Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-12-9198-10Tris buffer pH 7.4, 1000 ml 10 pouches £183.00£183.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
MC-12-9199-10Tris buffer pH 8.0, 1000 ml 10 pouches £183.00£183.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order
MC-12-9200-10Tris buffer pH 8.3, 1000 ml 10 pouches £183.00£183.00Offer until : 31-Aug-2019Offer Code : SUM19Starts 1st July 2019 View Offer Quantity Add to Order

Tris Buffer (Tris-HCl)

Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms

Medicago AB

Product description

Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms. Tris buffer is a good option for washing procedures in cell cultures (1) and is suitable as suspension buffer for biological samples.

Medicago’s Tris buffer is supplied as exactly pre-weighed powder in pouches at three different pH, 7.4, 8.0, and 8.3, one pouch giving 1000 ml of 1 M Tris-HCl buffer at 25°C.

Features

  • Formulated from analytical grade reagents
  • Choice of three different pre-set pH
  • Wide buffering capacity range: pH 7.0 to 9.2
  • Dissolve and go for greater convenience consistency from lot to lot

 

Directions for use

Empty one pouch of the Tris buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 300 ml of deionized water and stir the solution for a few minutes. Adjust the volume up to 1000 ml, stir until full dissolution and the buffer  is ready to use.

 

Shipping and storage

Tris buffer is shipped at room temperature. Store the pouches in a dry place at room temperature. Shelf life is three years after production date. Tris buffer solutions can be stored at room temperature or at 4°C for two weeks.

 

Specifications

Chemicals: Analytical grade

Format: Exactly pre-weighed powder mix

Concentration: 1 M Tris-HCl

Volume: 1000 ml

pH: 7.4, 8.0, and 8.3 ± 0.05 at 25°C

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Tris Buffer (Tris-HCl)

Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms

Medicago AB

References

1. Separation of three microbial amino acid polymerization factors (1996). J Lucas-Lenard, F Lipmann - National Academy os Sciences

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Tris Buffer (Tris-HCl)

Tris Buffer (Tris-HCl) pH 7.4, pH 8.0 and pH 8.3 Tris buffer is used in many applications within biochemistry and molecular biology laboratories and is a component in TAE and TBE (electrophoresis running buffer). Tris has a slightly alkaline buffering capacity between pH 7.0 and 9.2, which coincides with the typical physiological pH of most living organisms

Medicago AB

Applications

  • Component in TAE and TBE buffers
  • Cell and tissue culture procedures
  • Biochemistry and molecular biology

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200