Cambio - Excellence in Molecular Biology

Extraction & Purification - RNA

Extraction & Purification - RNA: Tissue/Cultures

RNA Isolation from tissues and cell cultures

Ribospin™ vRD (CE-marked)

Ribospin™ vRD provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. Ribospin™ vRD procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA and DNA isolation, instead of conventional alcohol precipitation or phenol/chloroform extraction. Ribospin™ vRD buffer system provides the effective binding condition of RNA and DNA to glassfiber membrane through mix with lysis and binding buffers. And then the impurities on the membrane are washed away by two different wash buffers.....

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Description

Ribospin™ vRD ( CE Marked)

 

Certificate of EU product notification:  Article 10 of  IVDD 98/79/EC

Description

Ribospin™ vRD provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. In contrast to conventional alcohol precipitation or phenol/chloroform extraction, the Ribospin™ vRD procedure employs glassfibre membrane technology for rapid and easy purification of high purity RNA and DNA. The Ribospin™ vRD buffer system provides optimal binding conditions for RNA and DNA to glassfibre membranes and enables impurities on the membrane to be effectively washed away by two different wash buffers. Pure RNA and DNA are eluted with nuclease-free water. The entire procedure takes only 20 minutes and the eluate is suitable for PCR, RT-PCR or any downstream application without further manipulation.

 Ribospin™ vRD plus! kit offers carrier RNA for purification of nucleic acid from very small amounts of sample.

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Features and Benefits

¦ Glassfibre membrane technology
¦ Sample size : up to 300 uL
¦ Preparation time : ~ 20 minutes
¦ Stable and consistent yield
¦ No phenol / chloroform extraction
¦ No ethanol precipitation
¦ Ready for use in PCR, RT-PCR, real-time PCR and other analytical procedures

 

 

 

 

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Procedure

Specification

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Protocols

RibospinTM vRD (plus!) Protocol

 

RibospinTM vRD Protocol

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References

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Applications & Benefits

Certificate of EU product notification:  Article 10 of  IVDD 98/79/EC

Ribospin VRD flyer.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200