Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Plasmid

Mini, midi and maxi preps including rapid plasmid prep kits.

Exfection™ Plasmid EF

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli......

GeneAll

Catalogue No.DescriptionPack SizePriceQty
GA-121-201GeneAll Exfection Plasmid EF 100 midi preps £980.00 Quantity Add to Order
GA-121-202GeneAll Exfection Plasmid EF 2 midi prep sample (max 3 per customer) £0.00 Quantity Add to Order
GA-121-220GeneAll Exfection Plasmid EF 20 midi preps £268.00 Quantity Add to Order
GA-121-930Buffer P3 100 ml £38.80 Quantity Add to Order
GA-121-940Buffer ER 15 ml £22.00 Quantity Add to Order
GA-121-950Buffer EG 150 ml £57.00 Quantity Add to Order

Exfection™ Plasmid EF

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli......

GeneAll

Description

ExfectionTM plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the
purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli.
It is a common contaminant in plasmid preparations and can significantly reduce transfection efficiencies, if not removed during DNA preparations. These kits use two methods for the removal of endotoxins: advanced phase separation and endotoxin removal washing. Endotoxin levels can be reduced to 0.1 EU/ug with ExfectionTM EF and to 10 EU/ug with ExfectionTM LE. Prepared plasmid DNA can be used for the transfection of most of cell lines in addition to most of molecular biological applications.

 

Features and Benefits

¦ Spin column format based on glassfiber membrane
¦ Convenient clearing of lysate with EzClearTM filter (Midi)
¦ High plasmid recoveries with high purity
¦ Fast preparation time and simple procedure
¦ High transfection efficiency in most cell-lines
¦ No need of additional materials
¦ No use of organic solvents

 

Procedure


 

Specification

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exfection™ Plasmid EF

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli......

GeneAll

Click here for protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Exfection™ Plasmid EF

Exfection™ plasmid LE (Low Endotoxin) and EF(Endotoxin-Free) provide simple and fast method for the purification of plasmid DNA with low endotoxin contaminants.Endotoxins (also known as lipopolysaccharides, LPS) are present in the cell membrane of gram-negative bacteria, such as Escherichia coli......

GeneAll

List of references here

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200