Cell Information:
Human Follicle Outer Root Sheath Cells (HORSC) are primary cells isolated from the outer root sheath of the hair follicle. Outer root sheath cells can be cultured in vitro to form epidermal equivalents, and can be used for autologous grafting. Furthermore, HORSC are reported to support stem cells in the bulge region of the outer sheath. These bulge cells are mutlipotent, critical for the self-renewal of hair follicles, and also have the capacity to regenerate sebaceous glands and epidermis.
The Transfection Reagent:
Transfection of primary cells, such as HORSC, has always been challenging, both technically and financially. The most common systems currently used for molecular delivery to these cells include expensive electroporation instruments and relatively toxic chemical formulations.
Human Follicle Outer Root Sheath Cell (HORSC) Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on HORSC. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for HORSC. No other transfection reagents can match the efficiency, convenience, and gentleness of Human Follicle Outer Root Sheath Cell (HORSC) Avalanche™ Transfection Reagent for transfection on HORSC. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in HORSC.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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| Data: |
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| FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on human follicle outer root sheath cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for human follicle outer root sheath cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Human Follicle Outer Root Sheath Cell Avalanche Transfection Reagent. |
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For Other Cells: |
Human Follicle Outer Root Sheath Cell (HORSC) Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.
Human Mesenchymal Stem Cell (Human Marrow Stromal Cell) (hMSC)
Human Hair Follicle Dermal Papilla Cell (HFDPC)
786-O Cell
Caki-1 Cell
MDCK Cell
Vero Cell
293 Cell
293T/17 Cell
Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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