Cell Culture Contamination Control: Detection
Mycoplasma Detection
As an efficient contamination control measure we recommend frequent routine testing of cell culture materials with Venor®Gem Mycoplasma Detection Kits. These PCR- and qPCR-based mycoplasma assays allow fast (within a few hours), simple, effective and sensitive detection of mycoplasma-contaminated cell cultures in any standard molecular biology laboratory.
Regular mycoplasma testing and control procedures can significantly help in avoiding the costly consequences of contamination ensuring safe biopharmaceuticals and reliable scientific results. Many scientific journals currently do not accept submissions of cell-based studies without evidence that mycoplasma testing has been performed.
To meet different customer needs Minerva Biolabs has developed a range of Venor®GeM mycoplasma detection assays using conventional PCR or qPCR methods which enable rapid and reliable identification of mycoplasma in cell cultures, culture supernatants and tissue culture media.
Conventional PCR
Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR.
Venor®GeM OneStep is a kit for direct detection. It requires minimal pipetting, the kit includes all reagents required for PCR in a ready-to-use lyophilized reaction mix. Only samples or controls need to be added prior to PCR.
Venor®GeM Advance is designed for direct detection. Designed to reduce the total assay time and the pipetting steps, the kit contains PCR strips of tubes, each pre-dispensed with all reagents necessary for each PCR reaction, including the polymerase. For additional convenience, the gel loading buffer and dye are already included in the reaction buffer. After PCR, the products can be loaded directly on an agarose gel.
Real-time PCR (qPCR)
Our qPCR kits are TaqMan®-based qPCR Assays with labelled probes: FAM® channel (mycoplasma) and HEX® channel (internal control to check for PCR inhibition from contaminants in sample). So not only does the qPCR approach remove a lot of hassle, but the internal controls also give you reassurance that your negatives are not false.
Venor®GeM qEP utilises quantitative, real-time PCR (qPCR) for fast (approx. 3 h) and reliable screening of cell culture supernatants for mycoplasma contamination. The kit can be used in combination with any type of real-time PCR cycler able to detect the fluorescence dyes FAM® and HEX®. For clinical diagnostics in which human samples are tested we recommend using this kit as it conforms to EP 2.6.7, USP <71> and JP G3. It is suitable for both research and industry where certified compliance is needed or desirable.
Venor®GeM qOneStep is a kit based on a real-time PCR (qPCR) assay for rapid, robust and sensitive detection of mycoplasma contamination. The detection procedure can be performed within 3 hours. Primers, nucleotides, probes, polymerase and an internal amplification control are provided as a ready-to-use, lyophilized reaction mix. This kit is a very straightforward and is highly suitable for academic labs and industrial applications where certified compliance of kits is not required.
AquaScreen® PCR Detection kit - detection of Pseudomonas aeruginosa
For the analysis of DNA extracts with qPCR. Kits include amplification reagents, polymerase and positive control (but not quantification standards). Quantification of Pseudomonas aeruginosa, compliant to AFNOR XP T 90-471 and ISO/TS 12869:2012.
Minerva Biolabs
Description
The AquaScreen® Pseudomonas aeruginosa qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® FastExtract procedure for quantification of Pseudomonas aeruginosa in water samples.
Recommended Use / Scope
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.
Kit Components
Freeze-dried primer, probes, nucleotides and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
Required Consumables
PCR reaction tubes
Optional: For calibration we recommend our Pseudomonas aeruginosa
DNA Calibration Reagent (Cat.-No. 52-0071).
Required lab devices
Pipetting equipment
qPCR cycler with filter sets for FAM™ and ROX™
Shelf Life and Storage
Components are maintainable at 2 to 8 °C for at least 12 months. After rehydratisation the reagents must be stored at -18 °C
Compliance
No guidelines are available for molecular testing of Pseudomonas aeruginosa in water samples.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Applications & Benefits
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200