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Transfection - Biontex

Transfection - Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

DOTAP™ Transfection Reagent

DOTAP™ Transfection Reagent is a well established transfection reagent for mammalian cells

Biontex

Catalogue No.DescriptionPack SizePriceQty
T010-1.0DOTAP™1ml £98.00£88.20Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order
T010-2.0DOTAP™2 x 1ml £159.00£143.10Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order
T010-5.0DOTAP™5x1ml £320.00£288.00Offer until : 31-Mar-2020Offer Code : BIO10 View Offer Quantity Add to Order

DOTAP™ Transfection Reagent

DOTAP™ Transfection Reagent is a well established transfection reagent for mammalian cells

Biontex

A well established transfection reagent for mammalian cells, DOTAP™ is a ready to use transfection reagent. It is a liposome formulation of a monocationic lipid (N-[1-(2,3-Dioleoyloxy)]-N,N,N-trimethylammonium propane methylsulphate in sterile water (concentration 1mg/ml).

There are a large number of reports in literature using this reagent (in vitro & in vivo). It combines reliability and efficiency with low costs. 1ml of DOTAP™ is usually sufficient for 50-100 transfections on 35mm dishes (6-well plates). DOTAP™ transfects nucleic acids both in the presence and absence of serum.

If stored at 4°C it is stable for at least 6 months

Structure of DOTAP™

 (C43H83NO8S, MW: 774,21)

First-generation transfection reagents

The unsatisfactory and unreliable results of the calcium phosphate transfection method led to the development of transfection reagents of the 1. Generation based on cationic lipids or liposomes.

These reagents are generally based on monocationic lipids with a quaternary ammonium ion. They do not feature buffering properties and therefore do not offer the option of endosome buffering, which explains their lower efficiency compared to modern reagents (e.g. METAFECTENE®). In addition, serum inhibition is reported for many of these transfection reagents with the exception of DOTAP.

A further feature of these reagents is a generally conventional transfection sequence providing for cell seeding and growth prior to the actual transfection and for change of medium after transfection. As a rule the proportion of DNA to lipids must be optimized carefully to ensure satisfactory results.

DOTAP was Biontex the starting point for the development of new transfection reagents. The temporal development series includes, in particular: METAFECTENE® < METAFECTENE® PRO < K2® Transfection System < K4® Transfection System. With the development degree the probability of a successful transfection increases (see also General Selection Aid).

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

DOTAP™ Transfection Reagent

DOTAP™ Transfection Reagent is a well established transfection reagent for mammalian cells

Biontex

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200