Metafectene® FluoR transfection reagent presents an outstanding method for visualizing the path of the carrier system through the eukaryotic cell and gaining new information from the transfection process, such as findings on the localization of specific components.
Metafectene® FluoR is based on second-generation transfection reagents, of which METAFECTENE® PRO is the best-proven example. The forward protocol used (24-hour incubation of seeded cells prior to transfection) ensures that cells reach the optimum condition for visualization.
The specific lipid composition of Metafectene® FluoR contains a covalent rhodamine B label which is designed not to impair the transfection process.
The two images below show cells transfected using Metafectene® FluoR and pCMV-eGFP. They clearly show the endocytotic uptake of red fluorescent lipoplexes in the cells and their perinuclear accretion. In addition, the superimposed shots of the fluorescence channels enable you to view the incipient expression of GFP by moving the cursor over the desired image.
Structure of rhodamine B label
Fluorescence spectrum of METAFECTENE® FluoR
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